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5-Ethynyl-UTP (5-EUTP)

5-Ethynyl-uridine-5'-triphosphate, Sodium salt

Cat. No. Amount Price (EUR) Buy / Note
CLK-T08-S 5 μl (100 mM) 130,84 Add to Basket/Quote Add to Notepad
CLK-T08-L 5 x 5 μl (100 mM) 523,35 Add to Basket/Quote Add to Notepad
CLK-T08-XL 50 μl (100 mM) 785,03 Add to Basket/Quote Add to Notepad
Structural formula of 5-Ethynyl-UTP (5-EUTP) (5-Ethynyl-uridine-5'-triphosphate, Sodium salt)
Structural formula of 5-Ethynyl-UTP (5-EUTP)

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C11H15N2O15P3 (free acid)

Molecular Weight: 508.16 g/mol (free acid)

Exact Mass: 507.97 g/mol (free acid)

Purity: ≥ 95 %

Form: clear aqueous solution

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 288 nm, ε 12.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription.
The resulting alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice

  • to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin)
  • to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes)
  • to crosslink the RNA to azide-functionalized biomolecules e.g.proteins
Presolski et al.[1] and Hong et al.[2] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

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Selected References:
[1] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[2] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.