HPLC Retention Time Standard
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months
CAS#: 118-00-3 (Guanosine), 5550-12-9 (GMP), 43139-22-6 (GDP), 36051-31-7 (GTP)
Concentration: 1 mM
The Guanosine Kit contains a set of 4 typical Guanosine analogs (Nucleoside, 5'-Mono, Di- and Triphosphate) all in one solution.
|Guanosine Analog|| CAS number|
GMP, sodium salt
GDP, sodium salt
GTP, sodium salt
Qualtiy Control (Fig. 2):
Each nucleoside (nucleotide) 1 mM in 100 mM Tris-HCl, pH 8.3
Absorbance: λmax 252 nm (pH 7); εmax 14.2 L mmol-1 cm-1
Guanosine-Nucleotide HPLC Retention Time Standard (10 μl) was analyzed by C18-Reversed Phase HPLC in 0.1 M TEAA buffer/acetonitrile pH 6.5. The four nucleotides are well separated with GMP eluting first (9.1 min) followed by GDP (9.8 min), GTP (10.6 min) and finally the most hydrophobic nucleoside Guanosine (11.1 min).