HPLC Retention Time Standard
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months
CAS#: 58-61-7 (Adenosine); 149022-20-8 (AMP); 20398-34-9 (ADP); 15237-44-2 (ATP)
Concentration: 1 mM
The Adenosine Kit contains a set of 4 typical Adenosine analogs (Nucleoside, 5'-Mono, Di- and Triphosphate) all in one solution.
Qualtiy Control (Fig. 2):
Each nucleoside (nucleotide) 1 mM in 100 mM Tris-HCl, pH 8.3
Absorbance: λmax 259 nm (pH 7); εmax 15.1 L mmol-1 cm-1
Adenosine-Nucleotide HPLC Retention Time Standard (10 μl) was analyzed by C18-Reversed Phase HPLC using 0.1 M TEAA buffer/acetonitrile pH 6.5. The four nucleotides are well separated with AMP eluting first (11.1 min) followed by ADP (11.7 min), ATP (12.3 min) and finally the most hydrophobic nucleoside Adenosine (14.0 min).