For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months
7-Methyl-Guanosines are important for RNA research. In living cells, mRNA is blocked ('capped') with a 7-Methyl-GTP residue at the 5' end. This prevents RNA degradation by 5'-exonucleases.
The 7-Methyl-Guanosine Kit contains a set of 4 typical 7-Methyl-Guanosine analogs (Nucleoside, 5'-Mono, Di- and Triphosphate).
|7-Methyl-Guanosine Analog||Cat. No.||Amount|
|m7GMP||NU-1135||100 μl (10 mM)|
|m7GDP||NU-1134||100 μl (10 mM)|
|m7GTP||NU-1122||100 μl (10 mM)|
Shen et al. (2001) Structural and thermodynamic behavior of eukaryotic initiation factor 4E in supramolecular formation with 4E-binding protein 1 and mRNA cap analogue, studied by spectroscopic Methods. Chem. Pharm. Bull. 49 (10):1299.
Carberry et al. (1989) A spectroscopic study of the binding of m7GTP and m7GpppG to human protein synthesis initiation factor 4E. Biochemistry 28 (20):8078.
Beemon et al. (1977) In vitro translation yields a possible Rous sarcoma virus src gene product. Proc. Natl. Acad. Sci. USA. 74 (8):3302.