For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months
5-Methyl-2'-deoxycytidines are used to inactivate restriction enzyme recognition sites and for gene expression and regulation studies.
The 5-Methyl-2'-deoxycytidine Kit contains a set of 4 typical 5-Methyl-2'-deoxycytidine analogs (Nucleoside, 5'-Mono, Di and Triphosphate).
|5-Methyl-2'-Deoxy-Cytidine Analog||Cat. No.||Amount|
|5-Methyl-dCMP||NU-1137||100 μl (10 mM)|
|5-Methyl-dCDP||NU-1163||100 μl (10 mM)|
|5-Methyl-dCTP||NU-1125||100 μl (10 mM)|
Kamiya et al. (2004) Important amino acids in the phosphohydrolase module of Escherichia coli Orf135. Biochem Biophys. Res. Commun. 323 (3):1063.
Holliday et al. (2002) DNA methylation and epigenetic inheritance. Methods 27 (2):179.
Kaito et al. (2001) Activation of the maternally preset program of apoptosis by microinjection of 5-aza-2'-deoxycytidine and 5-methyl-2'-deoxycytidine-5'-triphosphate in Xenopus laevis embryos. Dev. Growth Differ. 43 (4):383.
Wong et al. (1997) A novel method for producing partial restriction digestion of DNA fragments by PCR with 5-methyl-dCTP. Nucl. Acids Res. 25 (20):4169.
Chen et al. (1993) Direct induction of DNA hypermethylation in sea urchin embryos by microinjection of 5-methyl dCTP stimulates early histone gene expression and leads to developmental arrest. Dev. Biol. 155 (1):75.