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4-Thio-Uridine Kit

Cat. No. Amount Price (EUR) Buy / Note
NK-106 1 Kit 191,95 Add to Basket/Quote Add to Notepad
Structures of 6-Thio-Uridine analogs
Structures of 6-Thio-Uridine analogs

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C

Shelf Life: 12 months after date of delivery

Description:
4-Thio-Uridines are used for photochemical UV-crosslinking studies of RNA. Furthermore, they show altered hybridization properties compared to unmodified Uridine: 4-Thio-Uridine hybridizes to Guanosine instead of Adenosine.

The 4-Thio-Uridine Kit contains a set of 4 typical 4-Thio-Uridine analogs (Nucleoside, 5'-Mono, Di- and Triphosphate).

Content:

6-Thio-Uridine Analog Cat. No. Amount
4-Thio-U N-1043 1 mg
4-Thio-UMP NU-1154 50 μl (10 mM)
4-Thio-UDP NU-1155 50 μl (10 mM)
4-Thio-UTP NU-1156 50 μl (10 mM)

Selected References:
Zaher et al. (2006) A general RNA-capping ribozyme retains stereochemistry during cap exchange. J. Am. Chem. Soc. 128 (42):13894.
Kwon et al. (2001) DNA sequencing and genotyping by transcriptional synthesis of chain-terminated RNA ladders and MALDI-TOF mass spectrometry. Nucl. Acids Res. 29 (3):e11.
Testa et al. (1999) Thermodynamics of RNA-RNA Duplexes with 2-or 4-Thiouridines: Implications for Antisense Design and Targeting a Group I Intron. Biochemistry 38:16655.
Dontsova et al. (1994) Stem-loop IV of 5S rRNA lies close to the peptidyltransferase center. Proc. Natl. Acad. Sci. USA 91 (10):4125.
Sheng et al. (1993) Active site labeling of HIV-1 reverse transcriptase. Biochemistry 32 (18):4938.
Khanna et al. (1991) Photoaffinity labelling of the pea chloroplast transcriptional complex by nascent RNA in vitro. Nucl. Acids Res. 19 (18):4849.