5-Vinyl-2'-deoxyuridine (5-VdU)

For general laboratory use.

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₁H₁₄N₂O₅

Molecular Weight: 254.24 g/mol

Exact Mass: 254.09 g/mol

CAS#: 55520-67-7

Purity: ≥ 98 % (HPLC)

Form: solid

Color: white to off-white

Solubility: DMSO, methanol

Spectroscopic Properties: λ_max 292 nm, ε 9.2 L mmol^-1 cm^-1 (Methanol)

Applications:
DNA synthesis monitoring^[1]

Description:
5-VdU (5-Vinyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle.

5-VdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymidine. The resulting vinyl-functionalized DNA can subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging ^[1].

Related products:

• 5-Ethynyl-2'-deoxy-uridine (5-EdU), #CLK-N001
• 5-Vinyl-uridine (5-VU), #CLK-049

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Selected References:
[1] Rieder et al. (2014) Alkene-Tetrazine Ligation for Imaging Cellular DNA. Angew. Chem. Int. Ed. 53:9168.