5-Ethenyl-2'-deoxyuridine
2'-Deoxy-5-vinyluridine
2'-Deoxy-5-ethenyluridine
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| CLK-050-10 | 10 mg | 146,70 | Add to Basket/Quote Add to Notepad |
| CLK-050-50 | 50 mg | 505,90 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C11H14N2O5
Molecular Weight: 254.24 g/mol
Exact Mass: 254.09 g/mol
CAS#: 55520-67-7
Purity: ≥ 98 % (HPLC)
Form: solid
Color: white to off-white
Solubility: DMSO, methanol
Spectroscopic Properties: λmax 292 nm, ε 9.2 L mmol-1 cm-1 (Methanol)
Applications:
DNA synthesis monitoring[1]
Description:
5-VdU (5-Vinyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle.
5-VdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymidine. The resulting vinyl-functionalized DNA can subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging [1].
Related products:
BIOZ Product Citations:
Selected References:
[1] Rieder et al. (2014) Alkene-Tetrazine Ligation for Imaging Cellular DNA. Angew. Chem. Int. Ed. 53:9168.
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