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5-Bromo-2'-deoxyuridine (5-BrdU)

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5-Bromo-2'-deoxyuridine (5-BrdU) N-DN-1136-5G 5 g 233,38 Add to Basket/Quote Add to Notepad

For research use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C

Shelf Life: 30 months

Molecular Formula: C9H11BrN2O5

Molecular Weight: 307.10 g/mol

CAS#: 59-14-3

Purity: ≥ 98 % (HPLC)

Form: white crystalline powder

Solubility: methanol

Applications:
Used for detection of proliferating cells in living tissues. BrdU is efficiently incorporated into DNA of replicating cells by substituting thymidine. BrdU containing DNA is subsequently detected with BrdU-specific antibodies.

Selected References:
Gratzner et al. (1992) Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science 218:474.
Masterson et al. (2007) 5-Bromo-2-deoxyuridine activates DNA damage signalling responses and induces a senescence-like phenotype in p16-null lung cancer cells. Anticancer Drugs 18 (9):1053.
Morstyn et al. (1986) immunohistochemical identification of proliferating cells in organ culture using bromodeoxyuridine and a monoclonal antibody. J Histochem Cytochem 34 (6):697.
Qin et al. (1993) Comparison of the classical autoradiographic and the immunohistochemical methods with BrdU for measuring proliferation parameters in colon cancer. Anticancer Res 13:731.
Tapscott et al. (1989) 5-bromo-2'-deoxyuridine blocks myogenesis by extinguishing expression of MyoD1. Science 245 (4917):532.