For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 30 months
Molecular Formula: C9H11BrN2O5
Molecular Weight: 307.10 g/mol
Purity: ≥ 98 % (HPLC)
Form: white crystalline powder
Used for detection of proliferating cells in living tissues. BrdU is efficiently incorporated into DNA of replicating cells by substituting thymidine. BrdU containing DNA is subsequently detected with BrdU-specific antibodies.
Gratzner et al. (1992) Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science 218:474.
Masterson et al. (2007) 5-Bromo-2-deoxyuridine activates DNA damage signalling responses and induces a senescence-like phenotype in p16-null lung cancer cells. Anticancer Drugs 18 (9):1053.
Morstyn et al. (1986) immunohistochemical identification of proliferating cells in organ culture using bromodeoxyuridine and a monoclonal antibody. J Histochem Cytochem 34 (6):697.
Qin et al. (1993) Comparison of the classical autoradiographic and the immunohistochemical methods with BrdU for measuring proliferation parameters in colon cancer. Anticancer Res 13:731.
Tapscott et al. (1989) 5-bromo-2'-deoxyuridine blocks myogenesis by extinguishing expression of MyoD1. Science 245 (4917):532.