Adenosine triphosphate (ATP)-affinity chromatography has been widely used to purify various ATP-binding proteins such as kinases [1-4], β-and γ-glutamate decarboxylase and chaperones.
Efficient protein binding strongly depends on and the type of ATP-matrix attachment.
Try our ATP Affinity Test Kit or AMP Affinity Test Kit to find the most suitable material for the purification of your protein.
Ligand | Attachment Position | |
---|---|---|
Linked via the α-Phosphate | ||
α-Phosphate | ||
Linked via the γ-Phosphate | ||
γ-Phosphate | ||
γ-Phosphate | ||
γ-Phosphate | ||
γ-Phosphate | ||
γ-Phosphate | ||
γ-Phosphate | ||
Linked via the Nucleobase | ||
C-8 | ||
N-6 | ||
N-6 | ||
C-8 | ||
C-8 | ||
Linked via the Ribose | ||
2'/3'-OH | ||
2'/3'-OH | ||
2'-OH |
Bulk media based on Agarose (equals Sepharose™ 4B).
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