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Immobilized Adenosine Nucleotides

Adenosine triphosphate (ATP)-affinity chromatography has been widely used to purify various ATP-binding proteins such as kinases [1-4], β-and γ-glutamate decarboxylase and chaperones.

Efficient protein binding strongly depends on and the type of ATP-matrix attachment.

Try our ATP Affinity Test Kit or AMP Affinity Test Kit to find the most suitable material for the purification of your protein.

Ligand
Cat.-No.

Attachment Position
Linker Type

Linked via the α-Phosphate

α-Amino-hexyl-AMP
AC-158

α-Phosphate
6-atom carbon spacer

Linked via the γ-Phosphate

γ-Aminophenyl-ATP
AC-101

γ-Phosphate
10-atom carbon spacer

γ-Aminophenyl-ATP
AC-102

γ-Phosphate
without spacer

γ-Amino-octyl-ATP
AC-105

γ-Phosphate
8-atom carbon spacer

γ-Amino-hexyl-ATP
AC-116

γ-Phosphate
6-atom carbon spacer

γ-Amino-octyl-dATP
AC-111

γ-Phosphate
8-atom carbon spacer

γ-Amino-hexyl-dATP
AC-122

γ-Phosphate
6-atom carbon spacer

Linked via the Nucleobase

8-Amino-hexyl-ATP*
AC-127

C-8
6-atom carbon spacer

N6-(6-Amino)hexyl-ATP*
AC-129

N-6
6-atom carbon spacer

N6-(6-Amino)hexyl-AMP
AC-145

N-6
6-atom carbon spacer

8-Amino-hexyl-AMP
AC-156

C-8
6-atom carbon spacer

8-Amino-hexyl-cAMP
AC-146

C-8
6-atom carbon spacer

Linked via the Ribose

2'/3'-EDA-ATP*
AC-131

2'/3'-OH
EDA

2'/3'-EDA-AMP
AC-157

2'/3'-OH
EDA

2'-EDA-cAMP
AC-147

2'-OH
EDA

* Phosphorous chain may be degraded by NTP/dNTP hydrolyzing enzymes.


Also available: Auxilary Equipment → Proteins such as empty Columns, Spin Columns or Cartridges.

Bulk media based on Agarose (equals Sepharose™ 4B).
Sepharose™ is a trademark of GE Healthcare Companies.