Immobilized Adenosine Nucleotides

Adenosine triphosphate (ATP)-affinity chromatography has been widely used to purify various ATP-binding proteins such as kinases [1-4], β-and γ-glutamate decarboxylase and chaperones.

Efficient protein binding strongly depends on and the type of ATP-matrix attachment.

Try ourATP Affinity Test KitorAMP Affinity Test Kitto find the most suitable material for the purification of your protein.

Ligand Cat.-No.	Attachment Position Linker Type
Linked via the α-Phosphate
α-Amino-hexyl-AMP AC-158	α-Phosphate 6-atom carbon spacer
Linked via the γ-Phosphate
γ-Aminophenyl-ATP AC-101	γ-Phosphate 10-atom carbon spacer

γ-Aminophenyl-ATP AC-102	γ-Phosphate without spacer

γ-Amino-octyl-ATP AC-105	γ-Phosphate 8-atom carbon spacer

γ-Amino-hexyl-ATP AC-116	γ-Phosphate 6-atom carbon spacer

γ-Amino-octyl-dATP AC-111	γ-Phosphate 8-atom carbon spacer

γ-Amino-hexyl-dATP AC-122	γ-Phosphate 6-atom carbon spacer
Linked via the Nucleobase
8-Amino-hexyl-ATP* AC-127	C-8 6-atom carbon spacer

N⁶-(6-Amino)hexyl-ATP* AC-129	N-6 6-atom carbon spacer

N⁶-(6-Amino)hexyl-AMP AC-145	N-6 6-atom carbon spacer

8-Amino-hexyl-AMP AC-156	C-8 6-atom carbon spacer

8-Amino-hexyl-cAMP AC-146	C-8 6-atom carbon spacer
Linked via the Ribose
2'/3'-EDA-ATP* AC-131	2'/3'-OH EDA

2'/3'-EDA-AMP AC-157	2'/3'-OH EDA

2'-EDA-cAMP AC-147	2'-OH EDA

* Phosphorous chain may be degraded by NTP/dNTP hydrolyzing enzymes.

Bulk media based on Agarose (equals Sepharose™ 4B).
Sepharose™ is a trademark of GE Healthcare Companies.

Immobilized Adenosine Nucleotides

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