For research use only! Not intended for human or animal diagnostic or therapeutic uses.
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: Na3VO4
Molecular Weight: 183.91 g/mol
Activity: Inhibitor of ATPase, alkaline phosphatase and tyrosine phosphatase
Sodium Orthovanadate is an inhibitor of ATPase, alkaline phosphatase and tyrosine phosphatase that can be used in the purification procedure of ATP binding proteins (for further information see the manual of the ATP Affinity Test Kit #AK-102).
200 mM activated Sodium Orthvanadate dissolved in water, pH adjusted to 10.0
Dilute 1:1000 in purification buffers.
Haystead et al. (1993) Gamma-phosphate-linked ATP-Sepharose for the affinity purification of protein-kinases - rapid purification to homogeneity of sceletal-muscle mitogen-activated protein-kinase. Eur. J. Biochem. 214 (2):459.
Jenö et al. (1989) Purification and Charaterization of a 40 S Ribosomal Protein S6 Kinase from Vanadate-stimulated Swiss 3Τ3 Cells. J. Biol. Chem. 264:1293.
McNutt et al. (1981) Comparison of cell peripheries in the humancolonic adenocarcinoma cell lines SW480 and SW620 grown in floating chamber culture, cover slip culture, athymic (nude) mice, and BALB/c mice. Lab. Invest. 44:309.
Trayer et al. (1974) Affinity Chromatography of Nicotinamide Nucleotide-Dependent Dehydrogenases on Immobilized Nucleotide Derivates. Biochem. J. 139:609.
Scherer et al. (1954) Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix. J. Exp. Med. 97:695.