N6-(6-Amino)hexyl-dATP - Biotin
N6-(6-Amino)hexyl-2'-deoxyadenosine-5'-triphosphate - Biotin, Triethylammonium salt
Bio-7-dATP
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For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C26H43N8O14P3S (free acid)
Molecular Weight: 816.65 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: sterile clear aqueous solution, pH 7.5 ±0.5
Concentration: 1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 266 nm; ε 16.2 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation by terminal deoxynucleotidyl transferase[1]
Incorporation by nick translation[2]
Selected References:
[1] Flickinger et al. (1992) Differential incorporation of biotinylated nucleotides by terminal deoxynucleotidyl transferase. Nucleic Acids Res. 20:2382.
[2] Gebeyehu et al. (1987) Novel biotinylated nucleotide-analogs for labeling and colorimetric detection of DNA. Nucleic Acids Res. 15 (21):4513.