Biotin-X-5-Aminoallyl-uridine-5'-triphosphate, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C28H45N6O18P3S (free acid)
Molecular Weight: 878.67 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: sterile clear aequous solution in 10 mM Tris-HCl, pH 7.5 ±0.5
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 249/289 nm; ε 10.7/7.1 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Microarray based hybridization detection[1, 2]
 Lonergan et al. (2007) Comparison of target labeling methods for use of Affymetrix GeneChips. BMC Biotechnology 7:24.
 Glaid et al. (1989) Non-isotopic RNA probes. Comparison between different labels and detection systems. Histochemistry 93:91.
 Theissen et al. (1989) Degree of biotinylation in nucleic acids estimated by a gel retardation assay. Analyt. Biochem. 179:98.
 Baumann et al. (1988) Flow cytometric detection of ribosomal RNA in suspended cells by fluorescent in situ hybridization. Cytometry 9:517.