γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C28H44N7O16P3S (free acid)
Molecular Weight: 859.67 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: sterile clear aqueous solution in 10 mM Tris-HCl pH 7.5
Concentration: 1 mM - 1.1 mM
Spectroscopic Properties: λmax 294 nm; ε 9.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Biotin-11-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine.
Recommended Biotin-11-dCTP/dCTP ratio for PCR and Nick Translation: 50% Biotin-11-dCTP/ 50% dCTP
Please note: The optimal final concentration of Biotin-11-dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-11-dCTP/dCTP ratio is recommended.
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Bishop et al. (2008) APOBEC3G Inhibits Elongation of HIV-1 Reverse Transcripts. PLoS Pathogens 4:e1000231.