γ-[N-(Biotin-6-amino-hexanoyl-6-aminobutanoyl)]-5-(3-propargylamino)-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C32H51N8O17P3S (free acid)
Molecular Weight: 944.78 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: sterile clear aqueous solution in 10 mM Tris-HCl pH 7.5
Concentration: 1 mM - 1.1 mM
Spectroscopic Properties: λmax 294 nm; ε 9.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data
- Nick Translation with DNAse I/ DNA Polymerase I [1,2]
- Primer Extension with Klenow exo- 
- 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) 
- Reverse Transcription with MMLV Reverse Transcriptase 
Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine.
Recommended Biotin-16-dCTP/dCTP ratio for PCR: 50% Biotin-16-dCTP/ 50% dCTP
Please note: The optimal final concentration of Biotin-16-dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-16-dCTP/dTTP ratio is recommended.
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 Zammetto et al. (2005) Unambiguous identification of expressed MAGE-A genes on a DNA microarry. Clin Chem 51:2420.
 Grimmond et al. (2001) Expression Profiling with DNA Microarray's: A User's Perspective and Guide. In: DNA Microarrays: Gene Expression Applications (Jordan). Springer Verlag Berlin Heidelberg.