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5-Propargylamino-dCTP - solution

5-Propargylamino-2'-deoxycytidine-5'-triphosphate, Sodium salt

Product Cat. No. Amount Price (EUR) Buy / Note
5-Propargylamino-dCTP - solution NU-809S 10 μl (100 mM) 60,00 Add to Basket/Quote Add to Notepad
5-Propargylamino-dCTP - solution NU-809L 5 x 10 μl (100 mM) 280,00 Add to Basket/Quote Add to Notepad
5-Propargylamino-dCTP - solution NU-809XL 100 μl (100 mM) 420,00 Add to Basket/Quote Add to Notepad

Structural formula of 5-Propargylamino-dCTP - solution

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C12H19N4O13P3 (free acid)

Molecular Weight: 520.22 g/mol (free acid)

CAS#: 115899-39-3

Purity: ≥ 93 % (HPLC)

Form: slightly yellowish aqueous solution, pH 7.5 ±0.5

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 294 nm; ε 9.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Description:
Propargylamino-dCTP is recommended for two-step labeling of DNA e.g. by PCR. It is enzymatically incorporated into DNA as substitute for its natural counterpart dCTP. The resulting Amine-functionalized DNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice

  • to introduce a Biotin group (via NHS Ester of Biotin) for subsequent purification tasks
  • to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging

Applications:
Incorporation into DNA by

  • PCR with Taq polymerasein-house data