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Biotin-11-dUTP

Biotin-X-5-aminoallyl-dUTP

γ-[N-(Biotin-6-amino-hexanoyl)]-5-aminoallyl-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-803-BIOX-S 200 μl (1 mM) 84,79 Add to Basket/Quote Add to Notepad
NU-803-BIOX-L 5 x 200 μl (1 mM) 230,63 Add to Basket/Quote Add to Notepad
Structural formula of Biotin-11-dUTP (Biotin-X-5-aminoallyl-dUTP, γ-[N-(Biotin-6-amino-hexanoyl)]-5-aminoallyl-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt)
Structural formula of Biotin-11-dUTP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C28H45N6O17P3S (free acid)

Molecular Weight: 862.67 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: sterile clear aqueous solution, in 10 mM Tris-HCl pH 7.5 ±0.5

Concentration: 1 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 240/289 nm; ε 10.7/7.1 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase [1,2] & in-house data
- Nick Translation with DNAse I/ DNA Polymerase I [3,4] & in-house data
- Primer Extension with Klenow 3'-5' exo- [5]
- 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[6]
- Reverse Transcription with MMLV Reverse Transcriptase [7]

Description:
Biotin-11-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of uridine.

Recommended Biotin-11-dUTP/dTTP ratio for PCR and Nick Translation: 50% Biotin-11-dUTP/ 50% dTTP

Please note: The optimal final concentration of Biotin-11-dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-11-dUTP/dTTP ratio is recommended.

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Selected References:
[1] Day et al. (1990) Synthesis in vitro and application of biotinylated DNA probes for human papilloma virus type 16 by utilizing the polymerase chain reaction. Biochem J 267:119.
[2] Ried et al. (1992) Simultaneous visualization of severe different DNA probes by in situ hybridization using combinatorial fluorescence and digital imaging microscopy. Proc. Natl. Acad. Sci. USA 89:1388.
[3] Langer et al. (1981) Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78:6633.
[4] Brigati et al. (1983) Detection of viral genomes in cultured cells and para nembedded tissue sections using biotin-labled hybridization probes. Virology 126:32.
[5] Day et al. (2008) Microfluidic-based enzymatic on-chip labeling of miRNAs. N Biotechnol 25:142.
[6] Flickinger et al. (1992) Differential incorporation of biotinylated nucleotides by terminal deoxynucleotidyl transferase. Nucleic Acids Res 20:2382.
[7] Anderson et al. (2005) Incorporation of reporter-labeled nucleotides by DNA polymerases. Biotechniques 38:257.
Moritz et al. (2014) Simple methods for the 3' biotinylation of RNA. RNA. 20 (3):421.