γ-[N-(Biotin-6-amino-hexanoyl)]-5-aminoallyl-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.
Shelf Life: 12 months
Molecular Formula: C28H45N6O17P3S (free acid)
Molecular Weight: 862.67 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: sterile clear aqueous solution, in 10 mM Tris-HCl pH 7.5 ±0.5
Concentration: 1 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 240/289 nm; ε 10.7/7.1 L mmol-1 cm-1 (Tris-HCl pH 7.5)
DNA Labeling by PCR with Taq Polymerase (General Protocol I)[1,2]
DNA Labeling by Nick Translation with DNAse I/DNA Polymerase I[3,4]
DNA Labeling by Primer extension with Klenow 3'-5' exo-
3'-End DNA Labeling with Terminal deoxynucleotidyl Transferase (TdT)
cDNA Labeling by Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT)
 Day et al. (1990) Synthesis in vitro and application of biotinylated DNA probes for human papilloma virus type 16 by utilizing the polymerase chain reaction. Biochem J 267:119.
 Ried et al. (1992) Simultaneous visualization of severe different DNA probes by in situ hybridization using combinatorial fluorescence and digital imaging microscopy. Proc. Natl. Acad. Sci. USA 89:1388.
 Langer et al. (1981) Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78:6633.
 Brigati et al. (1983) Detection of viral genomes in cultured cells and para nembedded tissue sections using biotin-labled hybridization probes. Virology 126:32.
 Day et al. (2008) Microfluidic-based enzymatic on-chip labeling of miRNAs. N Biotechnol 25:142.
 Flickinger et al. (1992) Differential incorporation of biotinylated nucleotides by terminal deoxynucleotidyl transferase. Nucleic Acids Res 20:2382.
 Anderson et al. (2005) Incorporation of reporter-labeled nucleotides by DNA polymerases. Biotechniques 38:257.
Moritz et al. (2014) Simple methods for the 3' biotinylation of RNA. RNA. 20 (3):421.