Aminoallyl-dUTP - solution

5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, Sodium salt

Product Cat. No. Amount Price (EUR) Buy / Note
S pack NU-803S 10 μl (100 mM) 60,00 Add to Basket/Quote Add to Notepad
L pack NU-803L 5 x 10 μl (100 mM) 280,00 Add to Basket/Quote Add to Notepad
XL pack NU-803XL 100 μl (100 mM) 420,00 Add to Basket/Quote Add to Notepad

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C12H20N3O14P3 (free acid)

Molecular Weight: 523.22 g/mol (free acid)

CAS#: 116840-18-7

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution, pH 7.5 ±0.5

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 289 nm; ε 7.1 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Description:
Aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR, Nick Translation, Primer Extension, 3'-End Labeling and Reverse Transcription. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice

  • to introduce a Biotin group (via NHS Ester of Biotin) for subsequent purification tasks
  • to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging

Applications:
Incorporation into DNA/cDNA by

  • PCR with Taq polymerase[1], in-house data
  • Nick Translation with DNAse I/ DNA Polymerase I[2]
  • Primer Extension with Klenow exo- [3]
  • 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[4]
  • Reverse Transcription with MMLV Reverse Transcriptase[2]

Selected References:
[1] Dirsch et al. (2007) Probe production for in situ hybridization by PCR and subsequent covalent labeling with fluorescent dyes. Appl. Immunohistochem. Mol. Morphol. 3:332.
[2] Cox et al. (2004) Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling. BioTechniques 36:114.
[3] Cherkasov et al. (2010) New Nucleotide Analogues with Enhanced Signal Properties. Bioconjugate Chem. 21 (1):122.
[4] Unciti-Broceta et al. (2003) The use of solid supports to generate nucleic acid carriers.Accounts of Chemical Research 45:1140.