» Sign in

AP5G

P1-(5'-Adenosyl) P5-(5'-guanosyl) pentaphosphate, Triethylammonium salt

Product Cat. No. Amount Price (EUR) Buy / Note
AP5G NU-504S 50 μl (10 mM) 112,80 Add to Basket/Quote Add to Notepad
AP5G NU-504L 5 x 50 μl (10 mM) 330,42 Add to Basket/Quote Add to Notepad

Structural formula of AP5G

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C20H29N10O23P5 (free acid)

Molecular Weight: 932.37 g/mol (free acid)

CAS#: 56983-24-5

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution, pH 7.5 ±0.5

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 259 nm; ε 27.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Substrate replacement at UMP-CMP kinase[1]
Crystal structure of complex with GMP kinase[2]
Vasoconstriction via activation of P2X-receptors[3]
Activation of P2X receptor ion channels[4]

Specific Ligands:

Binding to purinoreceptors P2Y1 and P2X[5]

Binding to purinoreceptors P2X3 and rP2X1[6]

Selected References:
[1] Topalis et al. (2007) Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives -- a screening based on affinity for the UMP-CMP binding site. FEBS J. 274 (14):3704.
[2] Hible et al. (2006) Crystal structures of GMP kinase in complex with ganciclovir monophosphate and Ap5G. Biochimie 88:1157.
[3] van der Giet et al. (2001) The critical role of adenosine and guanosine in the affinity of dinucleoside polyphosphates to P (2X)-receptors in the isolated perfused rat kidney. Br. J. Pharmacol. 132 (2):467.
[4] Lewis et al. (2000)Effects of diadenosine polyphosphates (Ap (n)As) and adenosine polyphospho guanosines (Ap (n)Gs) on rat mesenteric artery P2X receptor ion channels.Br. J. Pharmacol. 129 (1):124.
[5] van der Giet et al. (2002) Effects of dinucleoside polyphosphates on regulation of coronary vascular tone. Eur. J. Pharmacol. 448 (2-3):207.
[6] Cinkilic et al. (2001) Selective agonism of group I P2X receptors by dinucleotides dependent on a single adenine moiety. J. Pharmacol. Exp. Ther. 299 (1):131.
Winward et al. (2010) Oxidation of the diphosphoinositol polyphosphate phosphohydrolase-like Nudix hydrolase Aps from Drosophila melanogaster induces thermolability--A possible regulatory switch? Int. J. Biochem. Cell Biol. 42 (7):1174.
Safrany et al. (2007) Characterisation of a bis (5'-nucleosyl)-tetraphosphatase (asymmetrical) from Drosophila melanogaster. Int. J. Biochem. Cell Biol. 39 (5):943.
Cherfils et al. (2006) Crystal structures of GMP kinase in complex with ganciclovir monophosphate and Ap5G. Biochimie 88 (9):1157.
Gross et al. (2006) Nucleotide-binding domains of Cystic Fibrosis Transmembrane Conductance Regulator, an ABC Transporter, Catalyze Adenylate Kinase Activity but not ATP Hydrolysis. J. Biol. Chem. 281 (7):4058.
Leslie et al. (2002) Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases. BMC Biochemistry 3:20.
Prinz et al. (1999) Binding of nucleotides to guanylate kinase, p21 (ras), and nucleoside-diphosphate kinase studied by nano-electrospray mass spectrometry. J. Biol. Chem. 274 (50):35337.
Ortiz et al. (1993) Specific synthesis of adenosine (5')tetraphospho (5')nucleoside and adenosine- (5')oligophospho (5')adenosine (n > 4) catalyzed by firefly luciverase. Eur. J. Biochem. 212 (1):263.
Feldhaus et al. (1975) Synthetic Inhibitors of Adenylate Kinases in the Assays for ATPases and Phosphokinases. Eur. J. Biochem. 57:197.