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AP4G

P1-(5'-Adenosyl) P4-(5'-guanosyl) tetraphosphate, Triethylammonium salt

Product Cat. No. Amount Price (EUR) Buy / Note
AP4G NU-503S 50 μl (10 mM) 112,80 Add to Basket/Quote Add to Notepad
AP4G NU-503L 5 x 50 μl (10 mM) 330,42 Add to Basket/Quote Add to Notepad

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C20H28N10O20P4 (free acid)

Molecular Weight: 852.39 g/mol (free acid)

CAS#: 10527-46-5

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution, pH 7.5 ±0.5

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 259 nm; ε 27.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Formation by T4 RNA ligase[1]
Formation by Pfu DNA ligase[2]
Acceptor substrate for T4 RNA ligase[3]

Selected References:
[1] Sillero et al. (2006) Synthesis of bisphosphonate derivatives of ATP by T4 RNA ligase. FEBS Lett. 580 (24):5723.
[2] Günther et al. (2002) Thermostable Pyrococcus furiosus DNA ligase catalyzes the synthesis of (di)nucleoside polyphosphates. Extremophiles. 6 (1):45.
[3] Atencia et al. (2000) Several dinucleoside polyphosphates are acceptor substrates in the T4 RNA ligase catalyzed reaction. Eur. J. Biochem. 267 (6):1707.
Winward et al. (2010) Oxidation of the diphosphoinositol polyphosphate phosphohydrolase-like Nudix hydrolase Aps from Drosophila melanogaster induces thermolability--A possible regulatory switch? Int. J. Biochem. Cell Biol. 42 (7):1174.
Safrany et al. (2007) Characterisation of a bis (5'-nucleosyl)-tetraphosphatase (asymmetrical) from Drosophila melanogaster. Int. J. Biochem. Cell Biol. 39 (5):943.
Leslie et al. (2002) Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases. BMC Biochemistry 3:20.
Ortiz et al. (1993) Specific synthesis of adenosine (5')tetraphospho (5')nucleoside and adenosine- (5')oligophospho (5')adenosine (n-greaterthan- 4) catalyzed by firefly luciverase. Eur. J. Biochem. 212 (1):263.
Palfi et al. (1991) Alterations in the accumulation of adenylylated nucleotides in heavy-metal-ion-stressed and heat-stressed Synechococcus sp strain pcc-6301, a cyanobacterium, in light and dark. Biochem. J. 276:487.
Brevet et al. (1991) Isolation and characterization of a dinucleoside triphosphatase from Saccharomyces-cerevisiae. J. Bacteriol. 173 (17):5275.
Sillero et al. (1991) Synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase. Eur. J. Biochem. 202 (2):507.
Avila et al. (1991) A paradoxical increase of a metabolite upon increased expression of its catabolic enzyme - the case of diadenosine tetraphosphate (AP4A) and AP4A phosphorylase-I in Saccharomyces-cerevisiae. J. Bacteriol. 173 (24):7875.
Garrison et al. (1984) Assay of adenosine 5'-P1-tetraphospho-P4-5'''-adenosine and adenosine 5'-P1-tetraphospho-P4-5'''-guanosine in Physarum-polycephalum and other eukaryotes - an isocratic high-pressure liquid-chromatography method. Biochem. J. 217 (3):805.
Barnes et al. (1984) Measurement of AP4A and AP4G during the cell-cycle of Physarumpolycephalum. H-S Z. Physiol. Chem. 365 (6):608.