2'-Deoxyadenosine-5'-[(α,β)-methyleno]triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C11H18N5O11P3 (free acid)
Molecular Weight: 489.21 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: clear aqueous solution, pH 7.5 ±0.5
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 259 nm; ε 15.4 L mmol-1 cm-1 (Tris-HCl pH 7.5)
X-ray analysis[1, 2]
 Batra et al. (2008) Structure of DNA polymerase β with active-site mismatches suggest a transient abasic site intermediate during misincorporation. Molecular Cell 30:315.
 Bakhtina et al. (2009) Contribution of the Reverse Rate of the Conformational Step to Polymerase # Fidelity. Biochemistry 48 (14):3197.
Kirby et al. (2012) Metal-Induced DNA Translocation Leads to DNA Polymerase Conformational Activation. Nucleic Acids Res. 40 (7):2974.
Jiang et al. (2011) Use of Chromophoric Ligands to Visually UNIT 7.15 Screen Co-Crystals of Putative Protein-Nucleic Acid Complexes. Curr. Protoc. Nucleic Acid Chem. 7 (7):15.1
Balbo et al. (2011) Kinetic mechanism of active site assembly and chemical catalysis of DNA polymerase β.Biochemistry 50 (45):9865.