dApCpp

(dAMPCPP)

2'-Deoxyadenosine-5'-[(α,β)-methyleno]triphosphate, Sodium salt

Product Cat. No. Amount Price (EUR) Buy / Note
S pack NU-432S 50 μl (10 mM) 124,37 Add to Basket/Quote Add to Notepad
L pack NU-432L 5 x 50 μl (10 mM) 364,30 Add to Basket/Quote Add to Notepad

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C11H18N5O11P3 (free acid)

Molecular Weight: 489.21 g/mol (free acid)

CAS#: 138998-91-1

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution, pH 7.5 ±0.5

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 259 nm; ε 15.4 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
X-ray analysis[1, 2]

Specific Ligands:

DNA-polymerase β[2]

Selected References:
[1] Batra et al. (2008) Structure of DNA polymerase β with active-site mismatches suggest a transient abasic site intermediate during misincorporation. Molecular Cell 30:315.
[2] Bakhtina et al. (2009) Contribution of the Reverse Rate of the Conformational Step to Polymerase # Fidelity. Biochemistry 48 (14):3197.
Kirby et al. (2012) Metal-Induced DNA Translocation Leads to DNA Polymerase Conformational Activation. Nucleic Acids Res. 40 (7):2974.
Jiang et al. (2011) Use of Chromophoric Ligands to Visually UNIT 7.15 Screen Co-Crystals of Putative Protein-Nucleic Acid Complexes. Curr. Protoc. Nucleic Acid Chem. 7 (7):15.1
Balbo et al. (2011) Kinetic mechanism of active site assembly and chemical catalysis of DNA polymerase β.Biochemistry 50 (45):9865.