Guanosine-5'-[(α,β)-methyleno]triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C11H18N5O13P3 (free acid)
Molecular Weight: 521.21 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: clear aqueous solution
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 252 nm; ε 13.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Atomic force microscopy
Assembly of microtubule
Dynamic of microtubule bundles
 Thomson et al. (2003) Large fluctuations in the disassembly rate of microtubules revealed by atomic force microscopy. Ultramicroscopy 97:239.
 Meurer-Grob et al. (2001) Microtubule structure at improved resolution. Biochemistry-US 40 (27):8000.
 Dixit et al. (2009) Microtubule plus-end tracking by CLIP-170 requires EB1. PNAS USA 106:492.
 Laan et al. (2008) Force-generation and dynamic instability of microtubule bundies. PNAS USA 105:8920.
 Shanker et al. (2007) Enhanced microtubule binding and tubulin assembly properties of conformationally constraint Paclitaxel derivatives. Biochemistry 46:11514.
 Ren et al. (2005) GTP cyclohydrolase II structure and mechanism. J. Biol. Chem. 280:36912.
Franck et al. (2010) Direct physical study of kinetochore-microtubule interactions by reconstitution and interrogation with an optical force clamp. Methods 51 (2):242.
Nitzsche et al. (2010) Studying kinesin motors by optical 3D-nanometry in gliding motility assays. Methods Cell. Biol. 95:247.
Gell et al. (2010) Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy. Methods Cell. Biol. 95:221.
Peters et al. (2010) Insight into the molecular mechanism of the multitasking kinesin-8 motor. EMBO J. 29 (20):3437.
Khrapunovich-Baine et al. (2009) Distinct Pose of Discodermolide in Taxol Binding Pocket Drives a Complementary Mode of Microtubule Stabilization. Biochemistry 48 (49):11677.
Nitzsche, et al. (2009) Quantum-dot-assisted characterization of microtubule rotations during cargo transport. Nature Nanotechnology 3:553.
Asbury et al. (2007) Tension applied through the Dam1 complex promotes microtubule elongation providing a direct mechanism for length control in mitosis. Nature Cell Biology 9:832.
Cary et al. (2005) Tonic and acute nitric oxide signaling through soluble guanylate cyclase is mediated by nonheme nitric oxide, ATP, and GTP. Nature Structural & Molecular Biology 102 (37) :13064.
Klaholz et al. (2005) Conformational transition of initiation factor 2 from the GTP- to GDP- bound state visualized on the ribosome. Nature Structural & Molecular Biology 11 :1145.
Li et al. (2000) Equilibrium studies of a fluorescent paclitaxel derivative binding to microtubules. Biochemistry-US 39 (3):616.
Lowe et al. (2000) Helical tubes of ftsz from Methanococcus jannaschii. Biol. Chem. 381 (9-10):993.
Muller-Reichert et al. (1998) Structural changes at microtubule ends accompanying GTP hydrolysis: information from a slowly hydrolyzable analogue of GTP, guanylyl (alpha,beta)methylenediphosphonate. Proc. Natl. Acad. Sci. USA 95 (7):3661.
Tran et al. (1997) A metastable intermediate state of microtubule dynamic instability that differs significantly between plus and minus ends. J. Cell Biol. 138 (1):105.
Tran et al. (1997) How tubulin subunits are lost from the shortening ends of microtubules. J. Struct. Biol. 118 (2):107.
Vulevic et al. (1997) Role of guanine nucleotides in the vinblastine-induced self-association of tubulin: effects of guanosine alpha,betamethylenetriphosphate and guanosine alpha,beta-methylenediphosphate. Biochemistry-US 36 (42):12828.
Caplow et al. (1996) Evidence that a single monolayer tubulin-GTP cap is both necessary and sufficient to stabilize microtubules. Mol. Biol. Cell 7 (4):663.
Hyman et al. (1995) Structural-changes accompanying GTP hydrolysis in microtubules - information from a slowly hydrolyzable analog guanylyl- (alpha,beta)-methylene-diphosphonate. J. Cell Biol. 128 (1-2):117.