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DMB-caged-ATP

Adenosine-5'-triphosphate, P3-(1-(3',5'-dimethoxyphenyl)-2-oxo-2-phenyl-ethyl)-ester, Triethylammonium salt

Product Cat. No. Amount Price (EUR) Buy / Note
DMB-caged-ATP NU-309S 10 μl (10 mM) 112,80 Add to Basket/Quote Add to Notepad
DMB-caged-ATP NU-309L 5 x 10 μl (10 mM) 330,42 Add to Basket/Quote Add to Notepad

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
store dark
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C26H30N5O16P3 (free acid)

Molecular Weight: 761.46 g/mol (free acid)

CAS#: 159899-51-1

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution, pH 7.5 ±0.5

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 256 nm; ε 25.6 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Ligand for purinergic receptors:
The nucleotide can be transported extra- or intracellular in a protected form to the target. After activation by well-defined conditions the liberated ATP can interact with P2X- and P2Y-receptors. Interacting subreceptor types and corresponding references are listed in Data sheet #NU-1010.
Agonistic ligand, mainly for nucleoside receptor A1
Nucleoside-triphosphates can be converted by different membrane-bound phosphatases into nucleosides acting as nucleoside receptor ligands. The caged form is protected during uptake and transport in animal experiments and can be well-directed released through activation at the target tissue.

Selected References:
Volonte et al. (2009) Membrane components and purinergic signalling: the purinome, a complex interplay among ligands, degrading enzymes, receptors and transporters. FEBS J. 276:318.
Yegutkin (2008) Nucleotide and nucleoside converting enzymes: Important modulators of purinergic signalling cascade. Biochim. Biophys. Acta 1783:673.
Apell et al. (1998) Partial reactions of the Na,K-ATPase: kinetic analysis and transport properties. Acta Physiol. Scand. 163:235.
Sokolov et al. (1998) Fast transient currents in Na,K-ATPase induced by ATP concentration jumps from the P-3-[1- (3',5'- dimethoxyphenyl)-2-phenyl-2-oxo]ethyl ester of ATP. Biophys. J. 74 (5):2285.
Sokolov et al. Fast transient currents in the Na,K-ATPase induced by ATP concentration jump experiments from DMBcaged ATP. Biophys. J. 72 (2):242.
Thirlwell et al. (1995) Inhibition of unloaded shortening velocity in permeabilized muscle-fibers by caged-ATP compounds. J. Muscle Res. Cell M. 16 (2):131.
Thirlwell et al. (1994) Kinetics of relaxation from rigor of permeabilized fast-twitch skeletal fibers from the rabbit using a novel caged-ATP and apyrase. Biophys. J. 67 (6):2436.
Corrie et al. (1992) Synthetic, mechanistic and photochemical studies of phosphate-esters of substituted benzoins. J. Chem. Soc. Perkin Trans. 1 (18):2409.
Corrie et al. (1992) The development and application of photosensitive caged compounds to aid time-resolved structure determination of macromolecules. Philos. T. Roy. Soc. A 340 (1657):233