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γ-(2-Azidoethyl)-ATP

γ-(2-Azidoethyl)-adenosine-5'-triphosphate, Sodium salt

Product Cat. No. Amount Price (EUR) Buy / Note
γ-(2-Azidoethyl)-ATP NU-1701S 100 μl (10 mM) 112,80 Add to Basket/Quote Add to Notepad
γ-(2-Azidoethyl)-ATP NU-1701L 5 x 100 μl (10 mM) 330,42 Add to Basket/Quote Add to Notepad

Structural formula of γ-(2-Azidoethyl)-ATP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C12H19N8O13P3 (free acid)

Molecular Weight: 576.25 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution, pH 7.5 ±0.5

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 259 nm; ε 15.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)

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Description:
Lee et al.[1] reported a non-radioactive version of in vitro phosphorylation were γ-[2-Azidoethyl]-ATP (compound 8[1]) has been successfully used instead of γ-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2.

The phosphorylated, azide-modified protein substrate can subsequently be labeled with Alkynes of biotin or fluorescent dyes via Cu(I)- catalyzed Click-Chemistry or DBCO-containing biotin or fluorescent dyes via Cu(I)-free Click-Chemistry.

Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

Applications:
in vitro phosphorylation of recombinant proteins[1]

Selected References:
[1] Lee et al. (2009) Synthesis and reactivity of novel γ-phosphate modified ATP analogues. Bioorg Med Chem Lett. 19:3804.
[2] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[3] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.