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2'-Fluoro-dATP

(2'F-dATP)

2'-Fluoro-2'-deoxyadenosine-5'-triphosphate, Sodium salt

Product Cat. No. Amount Price (EUR) Buy / Note
2'-Fluoro-dATP NU-151S 50 μl (100 mM) 87,13 Add to Basket/Quote Add to Notepad
2'-Fluoro-dATP NU-151L 5 x 50 μl (100 mM) 348,50 Add to Basket/Quote Add to Notepad

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C10H15N5O12P3F (free acid)

Molecular Weight: 509.17 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution, pH 7.5 ±0.5

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 259 nm; ε 15.4 L mmol-1 cm-1 (Tris-HCl pH 7.0)

Applications:
Substrate for ATP-requiring enzymes[1]
NMR-studies with F19[1]
Insertion sensitive NMR reporter group in RNA[2]
Inhibition of human DNA topoisomerases[3]

Selected References:
[1] Stockman (2008) 2-Fluoro-ATP as a versatile tool for 19F NMR-based activity screening. J. Am. Chem. Soc. 130 (18):5870.
[2] Scott et al. (2004) Enzymatic synthesis of 19F NMR studies of 2-fluoroadenine-substituted RNA. J. Am. Chem. Soc. 126 (38):11776.
[3] Liu et al. (1989) Interaction of several nucleoside triphosphate analogs and 10-hydroxycamptothecin with human DNA topoisomerases. Cancer Research 49 (6):1366.
Cho et al. (2003) Use of nucleotide analogs by class I and class II CCA-adding enzymes (tRNA nucleotidyltransferase): deciphering the basis for nucleotide selection. RNA 9 (8):970.
Rhie et al. (2003) Characterization of 2'-fluoro-RNA aptamers that bind preferentially to disease-associated conformations of prion protein and inhibit conversion. J. Biol. Chem. 278 (41):39697.
Sun et al. (2000) Catalytic nucleic acids: from lab to applications. Pharmacol. Rev. 52 (3):325.
Jayasena (1999) Aptamers: an emerging class of molecules that rival antibodies in diagnostics. Clin. Chem. 45 (9):1628.
Ono et al. (1997) 2'-Fluoro modified nucleic acids: polymerase-directed synthesis, properties and stability to analysis by matrix-assisted laser desorption/ionization mass spectrometry. Nucleic Acids Res. 25 (22):4581.