5-Bromo-dUTP

(5Br-dUTP)

5-Bromo-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt

5-BrdUTP

Product Cat. No. Amount Price (EUR) Buy / Note
S pack NU-122S 50 μl (10 mM) 118,45 Add to Basket/Quote Add to Notepad
L pack NU-122L 5 x 50 μl (10 mM) 346,95 Add to Basket/Quote Add to Notepad

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C9H14N2O14P3Br (free acid)

Molecular Weight: 547.04 g/mol (free acid)

CAS#: 102212-99-7

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution, pH 7.5 ±0.5

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 278 nm; ε 9.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Selected References:
Ma et al. (2008) The mutagenic properties of BrdUTP in a random mutagenesis process. Mol. Biol. Rep. 35:663.
Pal et al. (2002) Strong natural pausing by RNA polymerase II within 10 bases of transcription start may result in repeated slippage and reextension of the nascent RNA. Mol. Cell Biol. 22 (1):30.
Srinivasan et al. (2002) Characterization of RNA polymerase III transcription factor TFIIIC from the mulberry silkworm, Bombyx mori. Eur. J. Biochem. 26986):1780.
Mote et al. (1998) Recognition of a human arrest site is conserved between RNA polymerase II and prokaryotic RNA polymerases. J. Biol. Chem. 273 (27):16843.
Ekstrand (1996) A sensitive assay for the quantification of reverse transcriptase activity based on the use of carrier-bound template and non-radioactive-product detection, with special reference to human-immunodeficiency-virus isolation. Biotechnol Appl. Biochem. 23-2:95.
Gorczyca (1996) Laser scanning cytometer (LSC) analysis of fraction of labelled mitoses (FLM). Cell Prolif. 29 (10):539.
Li et al. (1995) Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation. Cell Prolif. 28 (11):571.
Ji et al. (1991) T4-phage ribonucleotide reductase - allosteric regulation invivo by thymidine triphosphate. J. Biol. Chem. 266 (25):16289.