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5-Bromo-UTP

(5Br-UTP)

5-Bromo-uridine-5'-triphosphate, Sodium salt

5-BrUTP

Product Cat. No. Amount Price (EUR) Buy / Note
5-Bromo-UTP NU-121S 50 μl (10 mM) 112,80 Add to Basket/Quote Add to Notepad
5-Bromo-UTP NU-121L 5 x 50 μl (10 mM) 330,42 Add to Basket/Quote Add to Notepad

Structural formula of 5-Bromo-UTP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C9H14N2O15P3Br (free acid)

Molecular Weight: 563.03 g/mol (free acid)

CAS#: 161848-60-8

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution, pH 7.5 ±0.5

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 278 nm; ε 9.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Agonist for P2Y2, P2Y4 and P2Y6 receptors[1,2,3]

Selected References:
[1] Wilson et al. (1998) Nucleotide-evoked calcium signals and anion secretion in equine cultured epithelia that express apical P2Y (2) receptors and pyrimidine nucleotide receptors. Brit. J. Pharmacol. 124 (4):832.
[2] Communi et al. (1996) Pharmacological characterization of the human P-2Y4 receptor. Eur. J. Pharmacol. 317 (2-3):383.
[3] El-Tayeb et al. (2006) Synthesis and structure-activity relationships of uracil nucleotide derivatives and analogues as agonists at human P2Y2, P2Y4, and P2Y6 receptors. J. Med. Chem. 49 (24):7076.
Gilles et al. (2007) Regulatory Mechanisms Differ in UMP Kinase from Gram-negative and Gram-positive Bacteria. J. Biol. Chem. 282 (10):7242.
Dunoyer et al. (2001) Peanut clump virus RNA-1-Encoded p15 regulates viral RNA accumulation but is not abundant at viral RNA replication sites. J. Virology 75 (4):1941.
Cmarko et al. (2000) Ultrastructural analysis of nucleolar transcription in cells microinjected with 5-bromo-UTP. Histochem. Cell Biol. 113 (3):181.
Cmarko et al. (1999) Ultrastructural analysis of transcription and splicing in the cell nucleus after bromo-UTP microinjection. Mol. Biol. Cell 10 (1):211.
Keene et al. (1999) Initially transcribed sequences strongly affect the extent of abortive initiation by RNA polymerase II. J. Biol. Chem. 274 (17):11526.
Wilson et al. (1999) Pyrimidine nucleotide-evoked inhibition of cyclic amp accumulation in equine epithelial cells. Exp. Physiol. 84 (4):639.
Mote et al. (1998) Recognition of a human arrest site is conserved between RNA polymerase II and prokaryotic RNA polymerases. J. Biol. Chem. 273 (27):16843.
Schul et al. (1998) Coiled bodies and U2 snRNA genes adjacent to coiled bodies are enriched in factors required for snRNA transcription. Mol. Biol. Cell 9 (5):1025.
Tanner et al. (1988) Binding interactions between yeast tRNA ligase and a precursor transfer ribonucleic acid containing two photoreactive uridine analogues. Biochemistry 27 (24):8852.