5-AcOHg-dCTP

5-Mercuriacetat-2'-deoxycytidine-5'-triphosphate, Sodium salt

Product Cat. No. Amount Price (EUR) Buy / Note
S pack NU-1149S 100 μl (10 mM) 112,80 Add to Basket/Quote Add to Notepad
L pack NU-1149L 5 x 100 μl (10 mM) 330,42 Add to Basket/Quote Add to Notepad

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C11H18HgN3O15P3 (free acid)

Molecular Weight: 725.78 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution, pH 7.5 ±0.5

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 272 nm; ε 9.1 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Selective isolation of Hg-DNA[1]
Substrate for polymerases[2]
Crystallization with heavy atom[2]

Selected References:
[1] Banfalvi et al. (1995) Effect of mercury substitution of DNA on its susceptibility to cleavage by restriction endonucleases. DNA Cell Biol. 14 (5):445.
[2] Dale et al. (1973) The Synthesis and Enzymatic Polymerization of Nucleotides Containing Mercury: Potential Tools for Nucleic Acid Sequencing and Structural Analysis. Proc. Natl. Acad. Sci. USA 70 (8):2238.