5-Methyl-2'-deoxycytidine-5'-triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.
Shelf Life: 12 months
Molecular Formula: C10H18N3O13P3 (free acid)
Molecular Weight: 481.18 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: clear aqueous solution, pH 7.5 ±0.5
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 277 nm; ε 9.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Analyzing methylation state of DNA
Cloning of identical sequences, FISH
X-ray of NTP-pyrophosphate hydrolase
Substrate specificity of Nudix hydrolase (Orf 135)
Incorporation by PCR
 Kaito et al. (2001) Activation of the maternally preset program of apoptosis by microinjection of 5-aza-2'-deoxycytidine and 5-methyl-2'-deoxycytidine-5'-triphosphate in Xenopus laevis embryos. Dev Growth Differ. 43 (4):383.
 Zabarovska et al. (2000) CIS-cloning of identical sequences between two complex genoms. Chromosome Research 8:77.
 Wu et al. (2007) Crystal structure of RS21-C6, involved in nucleoside triphosphate pyrophosphohydrolysis. J. Mol. Biol. 367:1405.
 Kamiya et al. (2004) Important amino acids in the phosphohydrolase module of Escherichia coli Orf135. Biochem Biophys Res Commun. 323 (3):1063.
 Dezhurov et al. (2005) A new highly efficient photoreactive analogue of dCTP. Synthesis, characterization, and application in photoaffinity modification of DNA binding proteins. Bioconjug. Chem. 16 (1):215.
Szwagierczak A et al. (2011) Characterization of PvuRts1I endonuclease as a tool to investigate genomic 5-hydroxymethylcytosine. Nucleic Acids Res. 39 (12):5149.
Frauer et al. (2009) A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding. Nucleic Acids Research 37 (3):e22.
Zeschnigk et al. (2009) Massive parallel bisulfite sequencing of CG-rich DNA fragments reveals that methylation of many X-chromosomal CpG islands in female blood DNA is incomplete. Human Molecular Genetics 18 (8):1439.
Holliday et al. (2002) DNA methylation and epigenetic inheritance. Methods. 27 (2):179.
Asamizu et al. (1999) A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Res. 6 (6):369.
Ito et al. (1998) Systematic method to obtain novel genes that are regulated by mi transcription factor: impaired expression of granzyme B and tryptophan hydroxylase in mi/mi cultured mast cells. Blood 91 (9):3210.
Lefaucheur et al. (1998) Evidence for three adult fast myosin heavy chain isoforms in type II skeletal muscle fibers in pigs. J. Anim. Sci. 76 (6):1584.
Wong et al. (1997) A novel method for producing partial restriction digestion of DNA fragments by PCR with 5-methyl-CTP. Nucleic Acids Res. 25 (20):4169.
Chen et al. (1993) Direct induction of DNA hypermethylation in sea urchin embryos by microinjection of 5-methyl dCTP stimulates early histone gene expression and leads to developmental arrest. Dev Biol. 155 (1):75.
Nelson et al. (1993) Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios. Nucl. Acid. Res. 21 (3):681.
Wong et al. (1991) PCR with 5-methyl-dCTP replacing dCTP. Nucl. Acid. Res. 19 (5):1081.