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5-Methyl-2'-deoxycytidine-5'-triphosphate, Sodium salt

Product Cat. No. Amount Price (EUR) Buy / Note
5-Methyl-dCTP NU-1125S 100 μl (10 mM) 118,45 Add to Basket/Quote Add to Notepad
5-Methyl-dCTP NU-1125L 5 x 100 μl (10 mM) 346,95 Add to Basket/Quote Add to Notepad

Structural formula of 5-Methyl-dCTP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C10H18N3O13P3 (free acid)

Molecular Weight: 481.18 g/mol (free acid)

CAS#: 22003-12-9

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution, pH 7.5 ±0.5

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 277 nm; ε 9.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Analyzing methylation state of DNA[1]
Cloning of identical sequences, FISH[2]
X-ray of NTP-pyrophosphate hydrolase[3]
Substrate specificity of Nudix hydrolase (Orf 135)[4]
Incorporation by PCR[5]
Pathological functions of epigenetic methylation[6,7]

Selected References:
[1] Kaito et al. (2001) Activation of the maternally preset program of apoptosis by microinjection of 5-aza-2'-deoxycytidine and 5-methyl-2'-deoxycytidine-5'-triphosphate in Xenopus laevis embryos. Dev Growth Differ. 43 (4):383.
[2] Zabarovska et al. (2000) CIS-cloning of identical sequences between two complex genoms. Chromosome Research 8:77.
[3] Wu et al. (2007) Crystal structure of RS21-C6, involved in nucleoside triphosphate pyrophosphohydrolysis. J. Mol. Biol. 367:1405.
[4] Kamiya et al. (2004) Important amino acids in the phosphohydrolase module of Escherichia coli Orf135. Biochem Biophys Res Commun. 323 (3):1063.
[5] Dezhurov et al. (2005) A new highly efficient photoreactive analogue of dCTP. Synthesis, characterization, and application in photoaffinity modification of DNA binding proteins. Bioconjug. Chem. 16 (1):215.
[6] Song et al. (2015) Human dCTP pyrophosphatase 1 promotes breast cancer cell growth and stemness through the modulation on 5-methyl-dCTP metabolism and global hypomethylation. Oncogenesis 4: e159.
[7] Lao et al. (2009) Incorporation of 5-chlorocytosine into mammalian DNA results in heritable gene silencing and altered cytosine methylation patterns. Carcinogenesis 30:886.
Szwagierczak A et al. (2011) Characterization of PvuRts1I endonuclease as a tool to investigate genomic 5-hydroxymethylcytosine. Nucleic Acids Res. 39 (12):5149.
Frauer et al. (2009) A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding. Nucleic Acids Research 37 (3):e22.
Zeschnigk et al. (2009) Massive parallel bisulfite sequencing of CG-rich DNA fragments reveals that methylation of many X-chromosomal CpG islands in female blood DNA is incomplete. Human Molecular Genetics 18 (8):1439.
Holliday et al. (2002) DNA methylation and epigenetic inheritance. Methods. 27 (2):179.
Asamizu et al. (1999) A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Res. 6 (6):369.
Ito et al. (1998) Systematic method to obtain novel genes that are regulated by mi transcription factor: impaired expression of granzyme B and tryptophan hydroxylase in mi/mi cultured mast cells. Blood 91 (9):3210.
Lefaucheur et al. (1998) Evidence for three adult fast myosin heavy chain isoforms in type II skeletal muscle fibers in pigs. J. Anim. Sci. 76 (6):1584.
Wong et al. (1997) A novel method for producing partial restriction digestion of DNA fragments by PCR with 5-methyl-CTP. Nucleic Acids Res. 25 (20):4169.
Chen et al. (1993) Direct induction of DNA hypermethylation in sea urchin embryos by microinjection of 5-methyl dCTP stimulates early histone gene expression and leads to developmental arrest. Dev Biol. 155 (1):75.
Nelson et al. (1993) Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios. Nucl. Acid. Res. 21 (3):681.
Wong et al. (1991) PCR with 5-methyl-dCTP replacing dCTP. Nucl. Acid. Res. 19 (5):1081.