5-Aza-2'-deoxycytidine-5'-triphosphate, Sodium salt
For research use only!
Unit Definition: 1 unit = 1 μl of a 10 mM solution
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.
Shelf Life: 12 months
Molecular Formula: C8H15N4O13P3 (free acid)
Molecular Weight: 468.14 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: clear aqueous solution, pH 7.5 ±0.5
Concentration: 10 mM
pH: 7.5 ±0.5
5-Aza-dCTP is a inhibitor of DNA methylation and an antileukemic agent. It has been used to reactivate silent tumor suppressor genes.
Determination of methyltransferase activity
 Frauer et al. (2009) A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding. Nucleic Acids Research 37 (3):e22.
Parker et al. (1987) Inhibition of DNA primase by nucleoside triphosphates and their arabinofuranosyl analogs. Mol. Pharmacol. 31 (2):146.
McIntosh et al. (1985) Synthesis and characterization of poly[d (G-z5C)]. B-Z transition and inhibition of DNA methylase. Biochemistry 24 (18):4806.
Momparler et al. (1984) Kinetic interaction of 5-AZA-2'-deoxycytidine-5'-monophosphate and its 5'-triphosphate with deoxycytidylate deaminase. Mol. Pharmacol. 25 (3):436.
Bouchard et al. (1983) Incorporation of 5-Aza-2'-deoxycytidine-5'-triphosphate into DNA. Interactions with mammalian DNA polymerase alpha and DNA methylase. Mol. Pharmacol. 24 (1):109.
Bouchard et al. (1983) Molecular Pharmacology 24-1:109-114.
Richard, L. et al. (2005) Anti-Cancer Drugs. 16-3:301-308.