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ddNTP Bundle

4 x 10 mM (ddATP, ddCTP, ddGTP, ddTTP)

2',3'-Dideoxyadenosine-5'-triphosphate, Lithium salt; 2',3'-Dideoxycytidine-5'-triphosphate, Lithium salt; 2',3'-Dideoxyguanosine-5'-triphosphate, Lithium salt; 2',3'-Dideoxythymidine-5'-triphosphate, Lithium salt

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Structural formula of ddNTP Bundle (4 x 10 mM (ddATP, ddCTP, ddGTP, ddTTP), 2',3'-Dideoxyadenosine-5'-triphosphate, Lithium salt; 2',3'-Dideoxycytidine-5'-triphosphate, Lithium salt; 2',3'-Dideoxyguanosine-5'-triphosphate, Lithium salt; 2',3'-Dideoxythymidine-5'-triphosphate, Lithium salt)
Structural formula of ddNTP Bundle

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula:
ddATP: C10H16N5O11P3 (free acid)
ddCTP: C9H16N3O12P3 (free acid)
ddGTP: C10H16N5O12P3 (free acid)
ddTTP: C10H17N2O13P3 (free acid)

Molecular Weight:
ddATP: 475.18 g/mol (free acid)
ddCTP: 451.16 g/mol (free acid)
ddGTP: 491.18 g/mol (free acid)
ddTTP: 466.17 g/mol (free acid)

Purity: ≥ 98 % (HPLC), functionally tested by chain termination sequencing reactions

Form: clear aqueous solution

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties:
ddATP: λmax 259 nm; ε 15.1 L mmol-1 cm-1 (Tris-HCl pH 7.5)
ddCTP: λmax 271 nm; ε 8.9 L mmol-1 cm-1 (Tris-HCl pH 7.5)
ddGTP: λmax 252 nm; ε 14.2 L mmol-1 cm-1 (Tris-HCl pH 7.5)
ddTTP: λmax 267 nm; ε 9.6 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Description:
ddNTP Bundle contains four separate solutions of ddATP, ddCTP, ddGTP, and ddTTP at a concentration of 10-11 mM supplied as clear aqueous solutions (pH 7.5 ±0.5).

ddATP (#NU-1015)
ddCTP (#NU-1016)
ddGTP (#NU-1017)
ddTTP (#NU-1018)

Selected References:
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463