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GTP - solution

100 mM

Guanosine 5'-triphosphate, Sodium salt

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GTP - solution NU-1012 1 ml (100 μmol) 27,58 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C

Shelf Life: 12 months

Molecular Formula: C10H16N5O14P3 (free acid)

Molecular Weight: 523.18 g/mol (free acid)

CAS#: 36051-31-7

Purity: > 99 % (HPLC)

Form: clear aqueous solution, pH 8.0 ±0.2 (4 °C)

Concentration: 100 mM ±2 %

pH: 8.0 ±0.2

Spectroscopic Properties: λmax 252 nm; ε 14.2 L mmol-1 cm-1 (Tris-HCl pH 7.0)

Description:
Ultrapure GTP supplied as clear aqueous solution (pH 8.0).

Applications:
Assembly of ribosomal units[1]
Microdomain formation by small GTPases[2]
Antiviral activity of large GTPases (dynamin superfamily)[3]
Regulation of exocytosis by Rho GTPases[4]
Mechanism of hydrolysis by ADP-ribosylation factors[5]

Specific Ligands:

Guanylate binding proteins[6]

Yeast septins[7]

Quality Control Specifications: in vitro transcription: suitable; contamination with bacterial and human DNA: not detectable; activity of DNase, Protease or Phosphatase: not detectable

Selected References:
[1] Blombach et al. (2011) Assembling the archeal ribosome: roles for transition factor-related GTPases. Biochemical Society Transactions 39:45.
[2] Stuermer (2011) Microdomain-forming proteins and the role of the reggies/flottilins during axon regeneration in zebrafish. Biochimica Biophysica Acta, Molecular Basis of Disease 1812:415.
[3] Haller et al. (2011) Human MxA protein: An Interferon-induced Dynamin-like GTPase with broad antiviral activity. J. Interferon and Cytokine Research 31:79.
[4] Stephane et al. (2011) Rho GTPases and exocytosis: what are the molecular links? Seminars in Cell and Developmental Biology 22:27.
[5] East et al. (2011) Models for the function of Arf GAPs. Seminars in Cell and Developmentan Biology 22:3.
[6] Vestal et al. (2011) The guanylate binding proteins: Emerging insights into the biochemical properties and functions of this family of large interferon-induced guanosine triphosphatase. J. Interferon and Cytokine Research 31:89.
[7] Younghoon et al. (2011) Septin structure and function in yeast and beyond. Trends in Cell Biology 21:141.
Drummond et al. (2011) Reconstitution and Organization of Escherichia coli Proto-ring Elements (FtsZ and FtsA) inside Giant Unilamellar Vesicles Obtained from Bacterial Inner Membranes. Methods Mol. Biol. 777 :29.
Katsuki et al. (2011) Preparation of dual-color polarity-marked fluorescent microtubule seeds. Methods Mol. Biol. 777:117.
Ramachandran et al. (2009) Membrane Insertion of the Pleckstrin Homology Domain Variable Loop 1 Is Critical for Dynamin-catalyzed Vesicle Scission. Molecular Biology of the Cell 20 (22):4630.