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dITP - Solution

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Structural formula of dITP - Solution
Structural formula of dITP - Solution

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C10H15N4O13P3 (free acid)

Molecular Weight: 492.16 g/mol (free acid)

CAS#: 16595-02-1

Purity: ≥ 99 % (HPLC)

Form: clear aqueous solution

Concentration: 100 mM - 110 mM

pH: 8.5 ±0.2 (22 °C)

Spectroscopic Properties: λmax 249 nm; ε 12.2 L mmol-1 cm-1 (Tris-HCl pH 7.0)

Description:
Ultrapure dITP supplied as sodium salt in purified water (pH 8.5).

Quality Control Specifications:
Low Copy Long Range PCR (18 kb, lambda DNA, template dilution series): PCR fragment with 50 pg of template or less
RT-PCR (749 bp fragment, human GAPDH gene, template dilution series): PCR fragment with 10 pg of template or less
Contamination with bacterial or human DNA: not detectable
DNases, RNases, Nicking Activity: not detectable
Proteases: not detectable

Selected References:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.