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dNTP Mix / 10 mM

Premix of 10 mM dATP, dCTP, dGTP and dTTP

2'-Deoxyadenosine 5'-triphosphate, Sodium salt; 2'-Deoxycytidine 5'-triphosphate, Sodium salt; 2'-Deoxyguanosine 5'-triphosphate, Sodium salt; 2'-Deoxythymidine 5'-triphosphate, Sodium salt

Product Cat. No. Amount Price (EUR) Buy / Note
dNTP Mix / 10 mM NU-1006S 200 μl 18,91 Add to Basket/Quote Add to Notepad
dNTP Mix / 10 mM NU-1006L 1 ml 75,65 Add to Basket/Quote Add to Notepad

Structural formula of dNTP Mix / 10 mM

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C

Shelf Life: 12 months

Molecular Formula: C10H13N5O12P3 (Anion); C9H13N3O13P3 (Anion); C10H13N5O13P3 (Anion); C10H14N2O14P3 (Anion)

Molecular Weight: 488.16 g/mol (Anion); 464.13 g/mol (Anion); 504.16 g/mol (Anion); 479.14 g/mol (Anion)

Purity: ≥ 99 % (HPLC)

Form: clear aqueous solution, pH 8.5 ±0.1 (4 °C)

pH: 8.5 ±0.1

Availability Restriction: Exclusively distributed in Japan by Greiner BioOne

dNTP Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP supplied as clear aqueous solution (pH 8.5).

For standard PCR applications a final concentration of 200 μM each dNTP is recommended.

Quality Control Specifications: 18 kb long range PCR (template dilution series): suitable; contamination with bacterial and human DNA: not detectable; activity of DNase, Protease or Phosphatase: not detectable

Selected References:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.