For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.
Shelf Life: 12 months
Molecular Formula: C10H16N5O13P3 (free acid)
Molecular Weight: 507.18 g/mol (free acid)
Purity: ≥ 99 % (HPLC)
Form: clear aqueous solution
Concentration: 100 mM - 110 mM
pH: 8.5 ±0.2 (22 °C)
Spectroscopic Properties: λmax 252 nm; ε 13.7 L mmol-1 cm-1 (Tris-HCl pH 7.0)
Ultrapure dGTP supplied as sodium salt in purified water (pH 8.5).
Quality Control Specifications:
Low Copy Long Range PCR (18 kb, lambda DNA, template dilution series): PCR fragment with 50 pg of template or less
RT-PCR (749 bp fragment, human GAPDH gene, template dilution series): PCR fragment with 10 pg of template or less
Contamination with bacterial or human DNA: not detectable
DNases, RNases, Nicking Activity: not detectable
Proteases: not detectable
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.
Availability Restriction: Exclusively distributed in Japan by Greiner BioOne