Featured Article - September 2025
A recent study[1] has revealed that FEN1 (Flap Endonuclease 1) is critical for the rapid repair of single-strand DNA breaks (SSBs) during the G1 phase of the cell cycle. SSBs are essentially nicks in one DNA strand, which can interfere with transcription or cause genome instability if not repaired fast. When cells are not yet dividing, FEN1 becomes the dominant repair pathway, ensuring that DNA integrity is safeguarded even before replication begins.
We are proud that our signature expertise in nucleotide chemistry contributed to the success of this study by Burdova et al.[1]. In their sSTRIDE assay, they incorporated biotinylated nucleotides from Jena Bioscience (Biotin-7-dATP, Biotin-16-dCTP, Biotin-16-dUTP and dGTP) to visualize and track DNA repair processes in living cells.
Why it matters:
- Most studies focus on SSBR in S or G2 phases (when DNA replication is active) or on other enzymes / pathways; this one shows how FEN1 is the central guardian of genome stability specifically in G1.
- It sharpens our understanding of DNA maintenance by rapid repair in non-dividing (or pre-division) cells
- Offers new angles for situations where cells spend a lot of time in G1 (slow-growing tissues, non-dividing cells) or for diseases where DNA repair is defective.
Read more: access the full article here!
Want to contribute with your own research in this field?
| Product | Cat. No. | Amount / Reactions | Price |
|---|---|---|---|
| Biotin-7-dATP | NU-835-BIO-S | 200 µl (1 mM) | 128,90 € |
| NU-835-BIO-L | 5 x 200 µl (1 mM) | 394,20 € | |
| Biotin-16-dCTP | NU-809-BIO16-S | 200 µl (1 mM) | 118,30 € |
| NU-809-BIO16-L | 5 x 200 µl (1 mM) | 290,80 € | |
| Biotin-16-dUTP | NU-803-BIO16-S | 200 µl (1 mM) | 104,30 € |
| NU-803-BIO16-L | 5 x 200 µl (1 mM) | 283,80 € | |
| dGTP | NU-1003L | 1 ml (100 mM) | 96,60 € |
| NU-1003-10ML | 10 ml (100 mM) | 675,90 € | |
| NU-1003-100ML | 100 ml (100 mM) | please inquire |
Reference:
[1] Burdova et al. (2025) FEN1 is critical for rapid single-strand break repair in G1 phase. Nucleic Acids Research 53:14; https://doi.org/10.1093/nar/gkaf710