» Sign in

Taq Pol

Thermostable DNA polymerase

Thermus aquaticus, recombinant, E. coli

Product Cat. No. Amount Price (EUR) Buy / Note
Taq Pol PCR-202S 200 units 35,00 Add to Basket/Quote Add to Notepad
Taq Pol PCR-202L 1000 units 140,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Unit Definition: One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmoles of dNTP's into an acid-insoluble form in 30 minutes at 70 °C using hering sperm DNA as substrate.

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Form: liquid

Concentration: 5 units/μl

Availability Restriction: Exclusively distributed in Japan by Greiner BioOne

Description:
Taq Pol is recommended for use in routine PCR reactions. It is optimized for high specificity and guarantees minimal by-product formation. The buffer system is recommended for plate based PCR and automated pipetting.
The enzyme replicates DNA at 72 °C. It catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exonuclease replacement activity but lacks a 3'→5' exonuclease activity.

Kit contents:
Taq Pol (red cap)
5 units/μl Taq DNA Polymerase in 20 mM Tris-HCl, 100 mM KCl,
0.1 mM EDTA, 1 mM DTT, 0.5 % Tween-20, 0.5 % Nonidet P-40,
50 % (v/v) Glycerol, pH 8.0 (25°C)

Taq Reaction Buffer complete (green cap)
10x conc.

200 mM Tris-HCl, 500 mM KCl, 15 mM MgCl2, pH 8.5 (25°C)

MgCl2 Stock Solution (yellow cap)
25 mM MgCl2

Recommended 50 μl PCR assay:

5 μl10x Taq Reaction Buffer completegreen cap
200 μMeach dNTP-
0.2 - 1 μMeach Primer-
2 - 50 ngtemplate DNA-
0.2 - 0.5 μl
(1 - 2.5 units)
Taq Polred cap
Fill up to 50 μlPCR-grade water-

,

Optimization of MgCl2 concentration:
A concentration of 1.5 mM Mg2+ is recommended for most applications. For an individual optimization add MgCl2 stock solution as shown in the table below.
50 μl PCR assay

MgCl2 Stock Solution1 μl2 μl3 μl5 μl
final MgCl2 conc.2 mM2.5 mM3 mM4 mM

Recommended cycling conditions:
initial
denaturation
94 °C2 min1x
denaturation94 °C30 sec30x
annealing1)45 - 68 °C30 sec30x
elongation2)72 °C30 sec - 4 min30x
final
elongation
72 °C2 min1x
1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kb is recommended.

For optimal specificity and amplification an individual optimization of the recommended parameters may be necessary for each new template DNA and/or primer pair.