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Taq Pol / high yield

Thermostable DNA polymerase

Thermus aquaticus, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PCR-201S 200 units 44,00 Add to Basket/Quote Add to Notepad
PCR-201L 1000 units 176,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Unit Definition: One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmoles of dNTP's into an acid-insoluble form in 30 minutes at 70 °C using hering sperm DNA as substrate.

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Form: liquid

Concentration: 5 units/μl

Taq Pol / high yield buffer is recommended for use in routine PCR reactions. The buffer system is optimized for high efficiency and gives superior amplification results in a broad range of reaction conditions with most primer-template pairs. The buffer system facilitates the incorporation of labeled or modified nucleotides into DNA. Note that the ammonium based buffer contains detergents and may interfere with automated pipetting systems.
The enzyme replicates DNA at 72 °C. It catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exo-nuclease replacement activity but lacks a 3'→5' exonuclease activity.

Taq Pol (red cap)
5 units/μl Taq DNA Polymerase in 20 mM Tris-HCl, 100 mM KCl,
0.1 mM EDTA, 1 mM DTT, 0.5 % Tween-20, 0.5 % Nonidet P-40,
50 % (v/v) Glycerol, pH 8.0 (25°C)

High Yield Buffer complete (green cap)
10x conc.

600 mM Tris-HCl, 150 mM (NH4)2SO4, 20 mM MgCl2,
0.05 % Tween-20, 0.05 % Nonidet P-40, pH 8.8 (25°C)

MgCl2 Stock Solution (yellow cap)
25 mM MgCl2

Recommended 50 μl PCR assay:

5 μl10x High Yield Buffer completegreen cap
200 μMeach dNTP-
0.2 - 1 μMeach Primer-
2 - 50 ngtemplate DNA-
0.2 - 0.5 μl
(1 - 2.5 units)
Taq Polred cap
Fill up to 50 μlPCR-grade water-

Optimization of MgCl2 concentration:
A concentration of 2.0 mM Mg2+ is recommended for most applications in combination with High Yield Buffer. For an individual optimization add MgCl2 stock solution as shown in the table below.
50 μl PCR assay

MgCl2 Stock Solution2 μl4 μl6 μl8 μl
final MgCl2 conc.3 mM4 mM5 mM6 mM

Recommended cycling conditions:
94 °C2 min1x
denaturation94 °C30 sec30x
annealing1)45 - 68 °C30 sec30x
elongation2)72 °C30 sec - 4 min30x
72 °C2 min1x
1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kb is recommended.

For optimal specificity and amplification an individual optimization of the recommended parameters may be necessary for each new template DNA and/or primer pair.

Availability Restriction: Exclusively distributed in Japan by Greiner BioOne