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Gel Loading Buffer with DNA Stain

Loading buffer for agarose or polyacrylamide gels with EvaGreen® fluorescent DNA stain

Product Cat. No. Amount Price (EUR) Buy / Note
Blue PCR-255-bl 5 x 1 ml 36,19 Add to Basket/Quote Add to Notepad
Green PCR-255-gr 5 x 1 ml 36,19 Add to Basket/Quote Add to Notepad
Orange PCR-255-or 5 x 1 ml 36,19 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
store dark

Shelf Life: 12 months

Form: Liquid

Concentration: 6x conc.

Jena Bioscience Gel Loading Buffers with DNA Stain are formulated to facilitate loading of DNA samples into the wells of agarose and polyacrylamide gels. The loading buffers contain EvaGreen® DNA Stain a fluorescent DNA intercalator dye specially developed for DNA analysis applications. High quantum yield and excellent stability makes EvaGreen® the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes Ethidium Bromide or SYBR® Green.
The buffers contain tracking dyes as indicator for DNA fragment migration. In addition, they contain glycerol to add density and EDTA to inhibit nuclease activities.
The buffers are optimized for loading of DNA fragments in a size range of:

Cat. No.Gel Loading Bufferfragment size
PCR-255-blBlue Gel Loading Bufferlarger than 500 bp
PCR-255-grGreen Gel Loading Bufferfrom 100 to 2000 bp
PCR-255-orOrange Gel Loading Buffersmaller than 500 bp

6x Gel Loading Buffer:
60 mM Tris-HCl (pH 7.5), 60 mM EDTA, 60 % (w/v) Glycerol, bromophenol blue (blue buffer), xylene cyanol FF (blue and green buffer), orange G (green and orange buffer), EvaGreen®

Add one part of Gel Loading Buffer to 5 parts of the DNA sample as shown in the table below. Spin down the tubes and vortex gently to achieve homogeneity.

DNA sample5 μl10 μl20 μl50 μl
6x Gel Loading Buffer1 μl2 μl4 μl10 μl

The Gel Loading Buffer provides highest convenience during routine handling and avoids commonly used gel staining procedures with Ethidium Bromide or SYBR® Green.

Fragment Separation on Agarose Gels:

DNA fragment sizeagarose gel conc.xylene cyanol FF running at approx.bromo-
phenol blue running
at approx.
orange G running at approx.
20-400 bp3.6 %280 bp40 bp2 bp
50-1000 bp3.0 %500 bp60 bp2 bp
100-2000 bp2.4 %900 bp100 bp3 bp
200-4000 bp1.8 %1800 bp40 bp5 bp
0.5-10 kb1.2 %4.5 kb0.5 kb10 bp
1-30 kb0.6 %12 kb1.2 kb100 bp