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PCR Control Kit

Amplification of a beta-actin gene fragment from human genomic DNA

Cat. No. Amount Price (EUR) Buy / Note
PCR-253 500 reactions 50,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles, aliquot genomic DNA
Short term storage (up to 1 week) at 4 °C possible.

Shelf Life: 12 months

Form: liquid

Related products:

  • Ready-to-use Mixes
  • Thermophilic Polymerase
  • Desoxynucleotides (dNTPs)
  • Custom Primers
  • DNA Ladders

Description:
PCR Control Kit is designed as tool to confirm or compare the quality and functionality of Taq DNA polymerases. The kit contains a forward and reverse primer-pair designed to amplify a 295 bp long fragment of the beta-actin gene from human genomic DNA.

Kit contents:
beta-actin Primer Mix (white cap)
500 μl, 15 μM each

Human genomic DNA (purple cap)
500 μl, 10 ng/μl

Recommended 50 μl PCR assay:

componentstock conc.final conc.amount
Reaction Buffer
complete
10x1x5 μl
dNTP Mix10 mM200 μM1 μl
beta-actin Primer Mix15 μM300 nM1 μl
Human
genomic DNA
10 ng/μl1-10 ng/assay0.1 - 1 μl
Taq
Polymerase
5 units/μl1.25 units/assay0.25 μl
PCR-grade water--fill up to
50 μl

Recommended cycling conditions:
initial
denaturation
95 °C2 min1x
denaturation95 °C10 sec30-35x
annealing59 °C20 sec30-35x
elongation72 °C30 sec30-35x
final
elongation
72 °C2 min1x