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PCR Additives Kit

Stabilizer and enhancer

Cat. No. Amount Price (EUR) Buy / Note
PCR-252 500 reactions 40,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C

Shelf Life: 12 months

Form: liquid

Description:
Taq-Stabilizer - 2x 1 ml

GC-Enhancer - 4x 1 ml

PCR Additives Kit contains a stabilizer and enhancer bundle for the amplification of difficult templates such as GC-rich or extremly long fragments.

Taq-Stabilizer:
5 - 10 % Taq-stabilizer in the PCR reaction is recommended to improve amplification efficiency and specificity. It stabilizes Taq polymerase and reduces secondary DNA structures.

Recommended 50 μl PCR assay:

componentstock conc.final conc.amount
Reaction Buffer
complete
10x1x5 μl
Taq-Stabilizer100 %5 - 10 %2.5 - 5 μl1)
dNTP Mix10 mM200 μM1 μl
Primer10 μM each200-400 nM1 - 2 μl
Template DNA-up to 50 ng-
Taq
Polymerase
5 units/μl0.025 units/μl0.25 μl
(1.25 units)
PCR-grade water--fill up to
50 μl
1) For optimal amplification an individual optimization of the recommended amounts may be necessary for each new template DNA and/or primer pair.
GC-Enhancer
10 - 20 % GC-enhancer in the PCR is recommended for amplifying GC-rich templates. It reduces the melting temperature of DNA. Thus, primer annealing temperature should be reduced by 1 - 2 °C.

Recommended 50 μl PCR assay:

componentstock conc.final conc.amount
Reaction Buffer
complete
10x1x5 μl
GC-Enhancer100 %10 - 20 %5 - 10 μl1)
dNTP Mix10 mM200 μM1 μl
Primer10 μM each200-400 nM1 - 2 μl
Template DNA-up to 50 ng-
Taq
Polymerase
5 units/μl0.025 units/μl0.25 μl
(1.25 units)
PCR-grade water--fill up to
50 μl
1) For optimal amplification an individual optimization of the recommended amounts may be necessary for each new template DNA and/or primer pair.

Availability Restriction: Exclusively distributed in Japan by Greiner BioOne