Red Load Taq Master / high yield

Master mix for direct gel loading

Product Cat. No. Amount Price (EUR) Buy / Note
S pack PCR-106S 1 ml (5x conc.) 45,00 Add to Basket/Quote Add to Notepad
L pack PCR-106L 5 x 1 ml (5x conc.) 180,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Form: liquid

Concentration: 5x conc.

Availability Restriction: Exclusively distributed in Japan by Greiner BioOne

Description:
Red Load Taq Master / high yield contains an inherent red dye and allows the direct loading of the PCR reaction product onto the gel. It contains all reagents required for PCR (except template and primer) in a premixed 5x concentrated ready-to-use solution.
The Master Mix is recommended for use in routine PCR reactions. It is optimized for high efficiency and gives superior amplification results in a broad range of reaction conditions with most primer-template pairs. Note that the mix is based on a detergent containing buffer system not recommended for plate based PCR and automated pipetting.
The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exonuclease replacement activity but lacks a 3'→5' exonuclease activity.
Kit contents:
5x Red Load Taq Master / high yield (red cap)
master mix of thermostable DNA polymerase, dATP, dCTP, dGTP, dTTP, (NH4)2SO4, MgCl2, Tween-20, Nonidet P-40, red dye, gel loading buffer and stabilizers.
PCR grade water (white cap)

Recommended 50 μl PCR assay:

10 μl5x Taq Master Mixred cap
0.2 - 1 μMeach Primer-
2 - 50 ngTemplate DNA-
Fill up to 50 μlPCR grade Waterwhite cap

Recommended cycling conditions:
Initial
denaturation
94 °C2 min1x
Denaturation94 °C30 sec30x
Annealing1)45 - 68 °C30 sec30x
Elongation2)72 °C30 sec - 3 min30x
Final
elongation
72 °C2 min1x
1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kbp is recommended.

For optimal specificity and amplification an individual optimization of the recommended parameters may be necessary for each new template DNA and/or primer pair.