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dNTP Mix / 25 mM

Premix of 25 mM dATP, dCTP, dGTP and dTTP

2'-Deoxyadenosine 5'-triphosphate, Sodium salt; 2'-Deoxycytidine 5'-triphosphate, Sodium salt; 2'-Deoxyguanosine 5'-triphosphate, Sodium salt; 2'-Deoxythymidine 5'-triphosphate, Sodium salt

Product Cat. No. Amount Price (EUR) Buy / Note
dNTP Mix / 25 mM NU-1023S 200 μl 24,05 Add to Basket/Quote Add to Notepad
dNTP Mix / 25 mM NU-1023L 1 ml 96,20 Add to Basket/Quote Add to Notepad

Structural formula of dNTP Mix / 25 mM

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C

Shelf Life: 12 months

Molecular Formula: C10H13N5O12P3 (Anion); C9H13N3O13P3 (Anion); C10H13N5O13P3 (Anion); C10H14N2O14P3 (Anion)

Molecular Weight: 488.16 g/mol (Anion); 464.13 g/mol (Anion); 504.16 g/mol (Anion); 479.14 g/mol (Anion)

Purity: ≥ 99 % (HPLC)

Form: clear aqueous solution, pH 8.5 ±0.1 (4 °C)

pH: 8.5 ±0.1

dNTP Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP supplied as clear aqueous solution (pH 8.5).

For standard PCR applications a final concentration of 200 μM each dNTP is recommended.

Quality Control Specifications: 18 kb long range PCR (template dilution series): suitable; contamination with bacterial and human DNA: not detectable; activity of DNase, Protease or Phosphatase: not detectable

Selected References:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.

Availability Restriction: Exclusively distributed in Japan by Greiner BioOne