Premix of 25 mM dATP, dCTP, dGTP and dTTP
2'-Deoxyadenosine 5'-triphosphate, Sodium salt; 2'-Deoxycytidine 5'-triphosphate, Sodium salt; 2'-Deoxyguanosine 5'-triphosphate, Sodium salt; 2'-Deoxythymidine 5'-triphosphate, Sodium salt
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Molecular Formula: C10H13N5O12P3 (Anion); C9H13N3O13P3 (Anion); C10H13N5O13P3 (Anion); C10H14N2O14P3 (Anion)
Molecular Weight: 488.16 g/mol (Anion); 464.13 g/mol (Anion); 504.16 g/mol (Anion); 479.14 g/mol (Anion)
Purity: ≥ 99 % (HPLC)
Form: clear aqueous solution, pH 8.5 ±0.1 (4 °C)
pH: 8.5 ±0.1
Availability Restriction: Exclusively distributed in Japan by Greiner BioOne
dNTP Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP supplied as clear aqueous solution (pH 8.5).
For standard PCR applications a final concentration of 200 μM each dNTP is recommended.
Quality Control Specifications: 18 kb long range PCR (template dilution series): suitable; contamination with bacterial and human DNA: not detectable; activity of DNase, Protease or Phosphatase: not detectable
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Gelfand et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.