Premix of 10 mM dATP, dCTP, dGTP and 20 mM dUTP
2'-Deoxyadenosine 5'-triphosphate, Sodium salt; 2'-Deoxycytidine 5'-triphosphate, Sodium salt; 2'-Deoxyguanosine 5'-triphosphate, Sodium salt; 2'-Deoxyuridine 5'-triphosphate, Sodium salt
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Molecular Formula: C10H13N5O12P3 (Anion); C9H13N3O13P3 (Anion); C10H13N5O13P3 (Anion); C9H12N2O14P3 (Anion)
Molecular Weight: 488.16 g/mol (Anion); 464.13 g/mol (Anion); 504.16 g/mol (Anion); 465.12 g/mol (Anion)
Purity: ≥ 99 % (HPLC)
Form: clear aqueous solution, pH 8.5 ±0.1 (4 °C)
pH: 8.5 ±0.1
dNTP Mix incl. dUTP is an mixture of 10 mM ultrapure dATP, dCTP, and dGTP, and 20 mM dUTP supplied as clear aqueous solution (pH 8.5). dUTP can be used in place of dTTP in PCR and RT-PCR protocols to prevent carry-over contaminations from previous amplifications.
Quality Control Specifications: 4 kb PCR (template dilution series): suitable; contamination with bacterial and human DNA: not detectable; activity of DNase, Protease or Phosphatase: not detectable
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.