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Taq Core Kit / high yield

Kit of thermostable DNA polymerase, dNTPs and high yield buffer

Product Cat. No. Amount Price (EUR) Buy / Note
Taq Core Kit / high yield PCR-231S 200 units 56,00 Add to Basket/Quote Add to Notepad
Taq Core Kit / high yield PCR-231L 1000 units 224,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Unit Definition: One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmoles of dNTP's into an acid-insoluble form in 30 minutes at 70 °C using hering sperm DNA as substrate.

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Form: liquid

Concentration: 5 units/μl

Related products:

  • Ready-to-Use Mixes / direct gel loading
  • Ready-to-Use Mixes
  • Thermophilic Polymerases
  • Deoxynucleotides (dNTPs)
  • Supplements
  • Primers and Oligonucleotides
  • DNA Ladders

Availability Restriction: Exclusively distributed in Japan by Greiner BioOne

Taq Core Kit / high yield contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. The premium quality polymerase, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results. The additional reaction buffer without MgCl2 in combination with the MgCl2 stock solution allows an easy optimization of difficult amplifications.
The kit is recommended for use in routine PCR reactions. It is optimized for high efficiency and gives best results in a broad range of reaction conditions with most primer-template pairs. The buffer system facilitates the incorporation of labeled or modified nucleotides into DNA. Note that the ammonium based buffer system contains detergent and is not recommended for plate based PCR and automated pipetting.
The enzyme replicates DNA at 72 °C. It catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exo-nuclease replacement activity but lacks a 3'→5' exonuclease activity.

Kit contents:
Taq Pol (red cap)
5 units/μl Taq DNA Polymerase in 20 mM Tris-HCl, 100 mM KCl,
0.1 mM EDTA, 1 mM DTT, 0.5 % Tween-20, 0.5 % Nonidet P-40,
50 % (v/v) Glycerol, pH 8.0 (25°C)

dNTP Mix (white cap)
10 mM each dNTP (dATP, dCTP, dGTP, dTTP)

High Yield Buffer complete (green cap)
10x conc.

600 mM Tris-HCl, 150 mM (NH4)2SO4, 20 mM MgCl2,
0.05 % Tween-20, 0.05 % Nonidet P-40, pH 8.8 (25°C)

MgCl2 Stock Solution (yellow cap)
25 mM MgCl2

Recommended 50 μl PCR assay:

5 μl10x High Yield Buffer completegreen cap
1 μldNTP Mixwhite cap
1-5 μleach Primer
(10 μM)
2 - 50 ngtemplate DNA-
0.2 - 0.5 μl
(1 - 2.5 units)
Taq Polred cap
Fill up to 50 μlPCR-grade water-

Optimization of MgCl2 concentration:
A concentration of 2.0 mM Mg2+ is recommended for most applications in combination with High Yield Buffer. For an individual optimization add MgCl2 stock solution as shown in the table below.
50 μl PCR assay
MgCl2 Stock Solution2 μl4 μl6 μl8 μl
final MgCl2 conc.3 mM4 mM5 mM6 mM

Recommended cycling conditions:
94 °C2 min1x
denaturation94 °C30 sec30x
annealing1)45 - 68 °C30 sec30x
elongation2)72 °C30 sec - 4 min30x
72 °C2 min1x
1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kb is recommended.

For optimal specificity and amplification an individual optimization of the recommended parameters may be necessary for each new template DNA and/or primer pair.