Spin column based RNA+DNA purification from blood, serum, tissue, cell culture or swabs
Cat. No. | Amount | Price (EUR) | Buy / Note |
---|---|---|---|
PP-235S | 50 preparations | 193,30 | Add to Basket/Quote Add to Notepad |
PP-235L | 250 preparations | 773,70 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at ambient temperature
Shelf Life: 12 months
Description:
Viral RNA+DNA Preparation Kit is designed for rapid and effective isolation of RNA and DNA from a variety of pathogen organisms such as virus or bacteria. Samples can be fresh or frozen plasma/blood (treated with anticoagulants except heparin), serum, other cell-free body fluids or pathogen-infected tissue. The kit allows high yield isolation of viral RNA/DNA from nasal or throat swabs.
The kit is specifically designed to isolate high-quality nucleic acids using low elution volumes and allowing sensitive downstream analysis including quantitative PCR and RT-PCR. The purified RNA/DNA is free of proteins and nucleases. Viral RNA+DNA Preparation Kit uses lysis buffer including chaotropic salts to inactivate RNases/DNases and advanced silica-gel membrane technology for fast purification of intact RNA/DNA. The preparation procedure is optimized to achieve reliable results within 30 min.
Content:
Component | PP-235S 50 Prep Kit | PP-235L 5 x 50 Prep Kit |
Lysis Buffer | 15 ml | 75 ml |
Ethanol (96-99 %) | fill up with 15 ml Ethanol | fill up with 75 ml Ethanol |
Washing Buffer A | 15 ml (add 15 ml Ethanol before use) | 75 ml (add 75 ml Ethanol before use) |
Washing Buffer B | 6.2 ml (add 25 ml Ethanol before use) | 31 ml (add 125 ml Ethanol before use) |
Elution Buffer | 3 ml | 15 ml |
Spin Columns and Collection Tubes | 50 spin columns and collection tubes | 5 x 50 spin columns and collection tubes |
Additional Materials Required:
96-99 % Ethanol
1.5 ml Tubes
Preparation procedure:
The DNA purification follows a cell lysis, RNA/DNA binding, washing and eluting procedure. Before starting, add Ethanol (96-99 %, not included in the kit) to Washing Buffer A and B as indicated on data sheet and bottles. Please note that the Ethanol concentration of a Washing Buffer may decrease during long term storage resulting in a drop-down of the final DNA yield.
The provided Lysis Buffer contains carrier molecules to enhance binding of RNA/DNA on the column membrane.
1a Preparation from nasal or throat swabs
1b Preparation from plasma, serum, urine, cell-culture supernantant, cell-free fluid or virus infected tissue
2 Column Loading
3 Column Washing
4 Elution
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