Solution based genomic DNA purification from yeast
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at ambient temperature
Shelf Life: 12 months
Yeast DNA Preparation Kit is designed for convenient and fast isolation of genomic DNA from yeast cells. The solution based system minimizes DNA fragmentation that may be problematic in other spin-column/filtration based method. Because phenol or chloroform is not used it is safe and does not produce any harmful waste.
Solution based genomic DNA purification kits guarantee minimal DNA fragmentation and yield DNA sized up to 150 kb.
Yields of genomic DNA will vary from sample to sample depending on the amount, quality and type of material processed. An amount of approx. 10 μg purified DNA per preparation can be expected.
Cell Resuspension Solution
Lyticase (before use, solve in Lyticase Suspension Solution to obtain a final concentration of 2.5 units/μl) - store at -20 °C
Lyticase Suspension Solution
Cell Lysis Solution
Protein Precipitation Solution
Washing Buffer (before use, add 96-99 % Ethanol as indicated on the bottle)
DNA Hydration Solution
RNase A (before use, solve in double distilled water to obtain a final concentration of 4 mg/ml) - store at -20 °C
To be provided by you:
Isopropanol (2-propanol) >99 %
96-99 % Ethanol
Microtubes 1.5 ml
Before start, provide >99 % Isopropanol (2-propanol) (not included in the kit).
For S pack (100 preps): Add 120 μl Lyticase Suspension Solution to the Lyticase tube, 200 μl dd-water to the RNase A tube and 48 ml
96-99 % Ethanol (not included in the kit) to the Washing Buffer bottle.
For L pack (500 preps): Add 120 μl Lyticase Suspension Solution to each Lyticase tube, 200 μl dd-water to each RNase A tube and 120 ml 96-99 % Ethanol (not included in the kit) to each Washing Buffer bottle.
|32 ml||160 ml|
|300 units||5x 300 units|
|130 μl||650 μl|
|32 ml||160 ml|
|11 ml||55 ml|
|Washing Buffer||add 48 ml Ethanol |
(final volume 60 ml)
|add 120 ml Ethanol to each bottle (final volume 150 ml each)|
|DNA Hydration Solution||11 ml||55 ml|
|0.8 mg||5x 0.8 mg|
1 Cell Lysis:
2 Protein Precipitation:
3 DNA Precipitation:
4 DNA Hydration:
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