Solution based genomic DNA purification from animal tissue and fungi
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at ambient temperature
Shelf Life: 12 months
Animal and Fungi DNA Preparation Kit is designed for convenient and fast isolation of genomic DNA from animal tissue and fungi. The solution based system minimizes DNA fragmentation that may be problematic in spin-column / filtration based methods. Because phenol or chloroform is not used it is safe and does not produce any harmful waste.
Solution based genomic DNA purification kits guarantee minimal DNA fragmentation and yield DNA sized up to 150 kb.
Cell Lysis Solution
Cell Resuspension Solution
Proteinase K (before use, solve in double distilled water to obtain a final concentration of 20 mg/ml) - store at -20 °C
Protein Precipitation Solution
Washing Buffer (before use, add 96-99 % Ethanol as indicated on the bottle)
DNA Hydration Solution
RNase A (before use, solve in double distilled water to obtain a final concentration of 4 mg/ml) - store at -20 °C
To be provided by you:
Isopropanol (2-propanol) >99 %
96-99 % Ethanol
Microtubes 1.5 ml
Before start, provide >99 % Isopropanol (2-propanol) (not included in the kit).
For S pack (100 preps): Add 200 μl dd-water to the Proteinase K tube, 200 μl dd-water to the RNase A tube and 48 ml 96-99 % Ethanol (not included in the kit) to the Washing Buffer bottle.
For L pack (500 preps): Add 200 μl dd-water to each Proteinase K tube, 200 μl dd-water to each RNase A tube and 120 ml 96-99 % Ethanol (not included in the kit) to each Washing Buffer bottle.
|32 ml||160 ml|
|32 ml||160 ml|
|4 mg||5x 4 mg|
|11 ml||55 ml|
|Washing Buffer||add 48 ml Ethanol |
(final volume 60 ml)
|add 120 ml Ethanol to each bottle (final volume 150 ml each)|
|DNA Hydration Solution||11 ml||55 ml|
|0.8 mg||5x 0.8 mg|
1a Cell Lysis for Animal Tissue:
1b Cell Lysis for Fungi:
2 Protein Precipitation:
3 DNA Precipitation:
4 DNA Hydration:
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