Solution based genomic DNA purification from bacteria
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at ambient temperature
Shelf Life: 12 months
Bacteria DNA Preparation Kit is designed for convenient and fast isolation of genomic DNA from gram-positive and gram-negative bacteria samples. The solution based system minimizes DNA fragmentation that may be problematic in spin-column / filtration based methods. Because phenol or chloroform is not used it is safe and does not produce any harmful waste.
Solution based genomic DNA purification kits guarantee minimal DNA fragmentation and yield DNA sized up to 150 kb.
Yields of genomic DNA will vary from sample to sample depending on the amount, quality and type of material processed. An amount of approx. 40 μg purified DNA per preparation can be expected.
Cell Resuspension Solution
Lysozyme (before use, solve in double distilled water to obtain a final concentration of 100 mg/ml) - store at -20 °C
Cell Lysis Solution
RNase A (before use, solve in double distilled water to obtain a final concentration of 4 mg/ml) - store at -20 °C
Protein Precipitation Solution
Washing Buffer (before use, add 96-99 % Ethanol as indicated on the bottle)
DNA Hydration Solution
To be provided by you:
Isopropanol (2-propanol) >99 %
96-99 % Ethanol
Microtubes 1.5 ml
Heating Block or Water Bath at 37 °C and 65 °C
Before start, provide >99 % Isopropanol (2-propanol) (not included in the kit).
For XS pack (20 preps): Add 50 μl dd-water to the Lysozyme tube,
40 μl dd-water to the RNase A tube and 9.6 ml 96-99 % Ethanol (not included in the kit) to the Washing Buffer bottle.
For S pack (100 preps): Add 250 μl dd-water to the Lysozyme tube, 200 μl dd-water to the RNase A tube and 48 ml 96-99 % Ethanol (not included in the kit) to the Washing Buffer bottle.
For L pack (500 preps): Add 250 μl dd-water to each Lysozyme tube, 200 μl dd-water to each RNase A tube and 120 ml 96-99 % Ethanol (not included in the kit) to each Washing Buffer bottle.
|6.4 ml||32 ml||160 ml|
|Lysozyme (100 mg/ml)||5 mg||25 mg||5x 25 mg|
|6.4 ml||32 ml||160 ml|
|0.16 mg||0.8 mg||5x 0.8 mg|
|2.2 ml||11 ml||55 ml|
|Washing Buffer||add 9.6 ml Ethanol |
(final volume 12 ml)
|add 48 ml Ethanol |
(final volume 60 ml)
|add 120 ml Ethanol to each bottle (final volume 150 ml each)|
|DNA Hydration Solution||2.2 ml||11 ml||55 ml|
1a Cell Lysis for Gram-Positive bacteria:
1b Cell Lysis for Gram-Negative Bacteria:
2 RNase Treatment:
3 Protein Precipitation:
4 DNA Precipitation:
5 DNA Hydration:
Availability Restriction: Exclusively distributed in Japan by Greiner BioOne